School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Neural Regen Res. 2014 Mar 1;9(5):474-80. doi: 10.4103/1673-5374.130063.
Tongluojiunao (TLJN) is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. However, its mechanism of action remains unclear. In the present study, primary cultured hippocampal neurons treated with Aβ1-42 (10 µmol/L) significantly increased the release of lactate dehydrogenase, which was markedly reduced by TLJN (2 µL/mL), specifically by the component geniposide (26 µmol/L), but not ginsenoside Rg1 (2.5 µmol/L). The estrogen receptor inhibitor, ICI182780 (1 µmol/L), did not block TLJN- or geniposide-mediated decrease of lactate dehydrogenase under Aβ1-42-exposed conditions. However, the phosphatidyl inositol 3-kinase or mitogen-activated protein kinase pathway inhibitor, LY294002 (50 µmol/L) or U0126 (10 µmol/L), respectively blocked the decrease of lactate dehydrogenase mediated by TLJN or geniposide. Therefore, these results suggest that the non-classical estrogen pathway (i.e., phosphatidyl inositol 3-kinase or mitogen-activated protein kinase) is involved in the neuroprotective effect of TLJN, specifically its component, geniposide, against Aβ1-42-mediated cell death in primary cultured hippocampal neurons.
通络救脑（TLJN）是一种草药，由栀子苷和人参皂苷 Rg1 两种主要成分组成。TLJN 已被证明可保护原代培养的海马神经元。然而，其作用机制尚不清楚。在本研究中，用 Aβ1-42（10µmol/L）处理的原代培养海马神经元显著增加了乳酸脱氢酶的释放，TLJN（2µL/mL），特别是栀子苷（26µmol/L），但不是人参皂苷 Rg1（2.5µmol/L）可明显减少乳酸脱氢酶的释放。雌激素受体抑制剂 ICI182780（1µmol/L）不能阻断 Aβ1-42 暴露条件下 TLJN 或栀子苷介导的乳酸脱氢酶降低。然而，磷脂酰肌醇 3-激酶或丝裂原活化蛋白激酶通路抑制剂 LY294002（50µmol/L）或 U0126（10µmol/L）分别阻断了 TLJN 或栀子苷介导的乳酸脱氢酶降低。因此，这些结果表明，非经典雌激素途径（即磷脂酰肌醇 3-激酶或丝裂原活化蛋白激酶）参与了 TLJN 的神经保护作用，特别是其成分栀子苷对原代培养海马神经元中 Aβ1-42 介导的细胞死亡的作用。
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