沉默 Nogo-66 受体基因的骨髓间充质干细胞修复脊髓损伤。

Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury.

机构信息

Department of Joint Orthopedics, Hebei Provincial Xingtai People's Hospital, Xingtai, Hebei Province, China.

Department of Spinal Orthopedics, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.

出版信息

Neural Regen Res. 2014 Apr 15;9(8):806-14. doi: 10.4103/1673-5374.131595.

DOI:10.4103/1673-5374.131595

PMID:25206893

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4146260/

Abstract

We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was significantly enhanced in the model group. After 8 weeks, the number of horseradish peroxidase-labeled nerve fibers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and significantly higher than in the model group. The newly formed nerve fibers and myelinated nerve fibers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.

摘要

我们假设，在骨髓间充质干细胞移植前通过 RNA 干扰沉默 Nogo-66 受体基因表达，可能会进一步改善脊髓横断损伤大鼠的神经功能。2 周后，沉默 Nogo-66 受体基因组的神经元和 BrdU 阳性细胞数量高于骨髓间充质干细胞组，与模型组相比差异有统计学意义。4 周后，模型组的行为表现明显改善。8 周后，沉默 Nogo-66 受体基因组的辣根过氧化物酶标记神经纤维数量高于骨髓间充质干细胞组，与模型组相比差异有统计学意义。在骨髓间充质干细胞组和沉默 Nogo-66 受体基因组的中央横断平面切片中可检测到新生神经纤维和髓鞘神经纤维。