原代培养的含有祖细胞的新生大鼠脉络丛，祖细胞能够分化。
Primary culture of choroid plexuses from neonate rats containing progenitor cells capable of differentiation.
机构信息
Department of Orthopaedics, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
Central Laboratory for Scientific Research, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
出版信息
Balkan Med J. 2013 Dec;30(4):350-4. doi: 10.5152/balkanmedj.2013.8259. Epub 2013 Dec 1.
BACKGROUND
The choroid plexuses, which could secrete a number of neurotrophins, have recently been used in transplantation in central nervous system diseases.
AIMS
To study the mechanism of nerve regeneration in the central nervous system by grafting choroid plexus tissues.
STUDY DESIGN
Animal experimentation.
METHODS
The choroid plexuses from the lateral ventricles of neonatal rats were cultured in adherent culture, and immunocytochemical methods were used to analyse the progenitor cells on days 2, 6, and 10 after seeding.
RESULTS
Expression of both nestin and glial fibrillary acidic protein was observed in small cell aggregates on day 2 in primary culture. Most of the nestin-positive cells on day 6 were immunoreactive to glial fibrillary acidic protein antibody. No cells expressing nestin or glial fibrillary acidic protein were seen on day 10.
CONCLUSION
These experimental results indicate that the choroid plexus contains a specific cell population - progenitor cells. Under in vitro experimental conditions, the progenitor cells differentiated into choroid plexus epithelial cells but did not form neurons or astrocytes.
背景
脉络丛可以分泌多种神经营养因子，最近已被用于中枢神经系统疾病的移植中。
目的
通过移植脉络丛组织来研究中枢神经系统神经再生的机制。
研究设计
动物实验。
方法
将新生大鼠侧脑室的脉络丛进行贴壁培养，分别于接种后第 2、6、10 天采用免疫细胞化学方法分析祖细胞。
结果
原代培养第 2 天可见到小细胞聚集物表达巢蛋白和胶质纤维酸性蛋白，第 6 天大多数巢蛋白阳性细胞对胶质纤维酸性蛋白抗体呈免疫反应性，第 10 天未见表达巢蛋白或胶质纤维酸性蛋白的细胞。
结论
这些实验结果表明脉络丛含有特定的细胞群体——祖细胞。在体外实验条件下，祖细胞分化为脉络丛上皮细胞，但不形成神经元或星形胶质细胞。
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