Hashemi Elham, Sadeghi Yousef, Aliaghaei Abbas, Seddighi Afsoun, Piryaei Abbas, Broujeni Mehdi Eskandarian, Shaerzadeh Fatemeh, Amini Abdollah, Pouriran Ramin
Department of Anatomy and Cell Biology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Shohada Tajrish Neurosurgical Center of Excellence, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Neural Regen Res. 2017 Jan;12(1):84-89. doi: 10.4103/1673-5374.198989.
As the key producer of cerebrospinal fluid (CSF), the choroid plexus (CP) provides a unique protective system in the central nervous system. CSF components are not invariable and they can change based on the pathological conditions of the central nervous system. The purpose of the present study was to assess the effects of non-traumatic and traumatic CSF on the differentiation of multipotent stem-like cells of CP into the neural and/or glial cells. CP epithelial cells were isolated from adult male rats and treated with human non-traumatic and traumatic CSF. Alterations in mRNA expression of and microtubule-associated protein (), as the specific markers of neurogenesis, and astrocyte marker glial fibrillary acidic protein () in cultured CP epithelial cells were evaluated using quantitative real-time PCR. The data revealed that treatment with CSF (non-traumatic and traumatic) led to increase in mRNA expression levels of and . Moreover, the expression of Nestin decreased in CP epithelial cells treated with non-traumatic CSF, while treatment with traumatic CSF significantly increased its mRNA level compared to the cells cultured only in DMEM/F12 as control. It seems that CP epithelial cells contain multipotent stem-like cells which are inducible under pathological conditions including exposure to traumatic CSF because of its compositions.
作为脑脊液（CSF）的关键产生者，脉络丛（CP）在中枢神经系统中提供了独特的保护系统。脑脊液成分并非一成不变，它们会根据中枢神经系统的病理状况而发生变化。本研究的目的是评估非创伤性和创伤性脑脊液对脉络丛多能干细胞样细胞向神经细胞和/或神经胶质细胞分化的影响。从成年雄性大鼠中分离出脉络丛上皮细胞，并用人类非创伤性和创伤性脑脊液进行处理。使用定量实时聚合酶链反应评估培养的脉络丛上皮细胞中作为神经发生特异性标志物的微管相关蛋白（）和神经干细胞标志物巢蛋白（）以及星形胶质细胞标志物胶质纤维酸性蛋白（）的信使核糖核酸表达变化。数据显示，用脑脊液（非创伤性和创伤性）处理导致和的信使核糖核酸表达水平增加。此外，用非创伤性脑脊液处理的脉络丛上皮细胞中巢蛋白的表达降低，而与仅在DMEM/F12中培养作为对照的细胞相比，用创伤性脑脊液处理显著增加了其信使核糖核酸水平。似乎脉络丛上皮细胞含有多能干细胞样细胞，由于其组成，这些细胞在包括暴露于创伤性脑脊液在内的病理条件下是可诱导的。
