Motokawa M, Kaku M, Matsuda Y, Kojima S, Sumi C, Kimura A, Tanimoto K
Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan,
Clin Oral Investig. 2015 May;19(4):929-35. doi: 10.1007/s00784-014-1310-6. Epub 2014 Sep 11.
The purpose of this study was to clarify whether occlusal hypofunction and its recovery affect the structure of the periodontal ligament (PDL) and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in rats.
Forty-eight Wistar rats aged 5 weeks were used and randomly divided into three groups: the hypofunctional group (HG), recovery group (RG), and control group (CG). In HG and RG, appliances were attached to the maxillary and mandibular incisors. In HG, appliances were set for 11 weeks. In RG, appliances were set for 7 weeks. Appliances were then removed at 0, 1, 3, 7, 14, and 28 days. Untreated rats served as CG. Histological sections were prepared and immunohistochemically stained for VEGF and bFGF. Three groups were evaluated for PDL area and the number of VEGF and bFGF immunopositive cells in PDL.
The number of immunopositive cells and PDL area in CG and RG were significantly larger when compared with HG, and PDL area in RG was similar to that in CG. In the recovery process, PDL area and number of VEGF-positive cells in PDL increased from days 0 to 7 and decreased from days 7 to 28. Conversely, the number of bFGF-positive cells in PDL increased significantly after day 1 and peaked at 28 days.
This study suggests that occlusal stimuli regulate PDL area through expression of VEGF and bFGF in rat PDL.
Occlusal stimuli are able to regulate the expression of VEGF and bFGF in PDL cells, and these growth factors may lead to alveolar bone remodeling in PDL.
本研究旨在阐明咬合功能减退及其恢复是否会影响大鼠牙周膜（PDL）的结构以及血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的表达。
选用48只5周龄的Wistar大鼠，随机分为三组：功能减退组（HG）、恢复组（RG）和对照组（CG）。HG组和RG组在上颌和下颌切牙上安装矫治器。HG组矫治器佩戴11周。RG组矫治器佩戴7周。然后在0、1、3、7、14和28天取下矫治器。未处理的大鼠作为CG组。制备组织学切片并对VEGF和bFGF进行免疫组织化学染色。评估三组的PDL面积以及PDL中VEGF和bFGF免疫阳性细胞的数量。
与HG组相比，CG组和RG组的免疫阳性细胞数量和PDL面积显著更大，且RG组的PDL面积与CG组相似。在恢复过程中，PDL面积和PDL中VEGF阳性细胞数量在第0天至第7天增加，在第7天至第28天减少。相反，PDL中bFGF阳性细胞数量在第1天后显著增加，并在第28天达到峰值。
本研究表明，咬合刺激通过大鼠PDL中VEGF和bFGF的表达来调节PDL面积。
咬合刺激能够调节PDL细胞中VEGF和bFGF的表达，这些生长因子可能导致PDL中的牙槽骨重塑。
