Reignoux Sabrina N A, Green Sarah, Ennos Richard A
Institute of Evolutionary Biology, University of Edinburgh, Ashworth Building, King's Buildings, Mayfield Rd., Edinburgh EH13 0AZ, United Kingdom.
Forest Research, Northern Research Station, Roslin, Midlothian EH25 9SY, United Kingdom.
Fungal Biol. 2014 Sep-Oct;118(9-10):835-45. doi: 10.1016/j.funbio.2014.07.002. Epub 2014 Jul 11.
The multi-locus phylogenetic species recognition approach and population genetic analysis of Amplified Fragment Length Polymorphism (AFLP) markers were used to delineate Lophodermium taxa inhabiting needles of Scots pine (Pinus sylvestris) in native pinewoods within Scotland. These analyses revealed three major lineages corresponding to the morphological species Lophodermium seditiosum and Lophodermium conigenum, fruiting on broken branches, and Lophodermium pinastri, fruiting on naturally fallen needles. Within L. pinastri three well supported sister clades were found representing cryptic taxa designated L. pinastri I, L. pinastri II, and L. pinastri III. Significant differences in mean growth rate in culture were found among the cryptic taxa. Taxon-specific primers based on ITS sequences were designed and used to classify over 500 Lophodermium isolates, derived from fallen needles of P. sylvestris in three Scottish and one French pinewood site, into the three L. pinastri cryptic taxa. Highly significant differences in the relative abundance of the three taxa were found among the Scottish pinewood sites, and between the French and all of the Scottish sites.
采用多位点系统发育物种识别方法和扩增片段长度多态性（AFLP）标记的群体遗传分析，来界定苏格兰本土松林中华山松（Pinus sylvestris）针叶上的散斑壳属分类群。这些分析揭示了三个主要谱系，分别对应于在断枝上结果的形态物种——有害散斑壳（Lophodermium seditiosum）和针叶散斑壳（Lophodermium conigenum），以及在自然掉落针叶上结果的欧洲散斑壳（Lophodermium pinastri）。在欧洲散斑壳内，发现了三个得到充分支持的姐妹分支，代表了被指定为欧洲散斑壳I、欧洲散斑壳II和欧洲散斑壳III的隐存分类群。在这些隐存分类群中，发现培养条件下的平均生长速率存在显著差异。基于ITS序列设计了分类群特异性引物，并用于将来自三个苏格兰和一个法国松树林地中华山松掉落针叶的500多个散斑壳分离株，分类为三个欧洲散斑壳隐存分类群。在苏格兰松树林地之间，以及法国和所有苏格兰林地之间，发现这三个分类群的相对丰度存在极显著差异。
