Bornot Julie, Aceves-Lara César-Arturo, Molina-Jouve Carole, Uribelarrea Jean-Louis, Gorret Nathalie
Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, 31077, Toulouse, France.
Extremophiles. 2014 Nov;18(6):1009-21. doi: 10.1007/s00792-014-0671-8. Epub 2014 Sep 11.
Few studies concerning the nutritional requirements of Deinococcus geothermalis DSM 11300 have been conducted to date. Three defined media compositions have been published for the growth of this strain but they were found to be inadequate to achieve growth without limitation. Furthermore, growth curves, biomass concentration and growth rates were generally not available. Analysis in Principal Components was used in this work to compare and consequently to highlight the main compounds which differ between published chemically defined media. When available, biomass concentration, and/or growth rate were superimposed to the PCA analysis. The formulations of the media were collected from existing literature; media compositions designed for the growth of several strains of Deinococcaceae or Micrococcaceae were included. The results showed that a defined medium adapted from Holland et al. (Appl Microbiol Biotechnol 72:1074-1082, 2006) was the best basal medium and was chosen for further studies. A growth rate of 0.03 h(-1) and a final OD600nm of 0.55 were obtained, but the growth was linear. Then, the effects of several medium components on oxygen uptake and biomass production by Deinococcus geothermalis DSM 11300 were studied using a respirometry-based method, to search for the nutritional limitation. The results revealed that the whole yeast extract in the medium with glucose is necessary to obtain a non-limiting growth of Deinococcus geothermalis DSM 11300 at a maximum growth rate of 0.64 h(-1) at 45 °C.
迄今为止，针对嗜热栖热放线菌DSM 11300营养需求的研究很少。已发表了三种用于该菌株生长的确定培养基成分，但发现它们不足以实现无限制生长。此外，生长曲线、生物量浓度和生长速率通常也无法获得。本研究采用主成分分析来比较并突出已发表的化学成分确定培养基之间不同的主要化合物。如有可用数据，将生物量浓度和/或生长速率叠加到主成分分析中。培养基配方取自现有文献；包括为几种嗜热放线菌科或微球菌科菌株生长设计的培养基成分。结果表明，一种改编自Holland等人（《应用微生物学与生物技术》72:1074 - 1082，2006年）的确定培养基是最佳基础培养基，并被选用于进一步研究。获得了0.03 h⁻¹的生长速率和0.55的最终OD600nm，但生长是线性的。然后，使用基于呼吸测定法研究了几种培养基成分对嗜热栖热放线菌DSM 11300氧气摄取和生物量产生的影响，以寻找营养限制因素。结果表明，含有葡萄糖的培养基中的全酵母提取物对于嗜热栖热放线菌DSM 11300在45℃下以0.64 h⁻¹的最大生长速率实现无限制生长是必要的。
