Thiagarajan Venkata R K, Shanmugam Palanichamy, Krishnan Uma M, Muthuraman Arunachalam
School of Chemical and Biotechnology, Sastra University, Tamilnadu, India.
Department of Pharmaceutical Sciences & Research, Sankaralingam Bhuvaneshwari College of Pharmacy, Tamilnadu, India.
An Acad Bras Cienc. 2014 Sep;86(3):1435-50. doi: 10.1590/0001-3765201420130404. Epub 2014 Sep 9.
The aim of the present study is to investigate the ameliorative potential of ethanolic extract of whole plant of Vernonia cinerea in the chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain in rats. Behavioral parameters such as a hot plate, acetone drop, paw pressure, Von Frey hair and tail immersion tests were performed to assess the degree of thermal, chemical and mechanical hyperalgesia and allodynia. Biochemical changes in sciatic nerve tissue were ruled out by estimating thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total calcium levels. Ethanolic extract of Vernonia cinerea and pregabalin were administered for 14 consecutive days starting from the day of surgery. CCI of sciatic nerve has been shown to induce significant changes in behavioral, biochemical and histopathological assessments when compared to the sham control group. Vernonia cinerea attenuated in a dose dependent manner the above pathological changes induced by CCI of the sciatic nerve, which is similar to attenuation of the pregabalin pretreated group. The ameliorating effect of ethanolic extract of Vernonia cinerea against CCI of sciatic nerve induced neuropathic pain may be due to the presence of flavonoids and this effect is attributed to anti-oxidative, neuroprotective and calcium channel modulator actions of these compounds.
本研究的目的是探讨灰叶斑鸠菊全草乙醇提取物对大鼠坐骨神经慢性压迫损伤（CCI）所致神经性疼痛的改善潜力。通过进行热板、丙酮滴注、爪部压力、von Frey毛发和尾部浸入试验等行为学参数评估，以确定热、化学和机械性痛觉过敏及异常性疼痛的程度。通过测定硫代巴比妥酸反应物质（TBARS）、还原型谷胱甘肽（GSH）和总钙水平，排除坐骨神经组织中的生化变化。从手术当天开始，连续14天给予灰叶斑鸠菊乙醇提取物和普瑞巴林。与假手术对照组相比，坐骨神经CCI已显示出在行为、生化和组织病理学评估方面的显著变化。灰叶斑鸠菊以剂量依赖性方式减轻了坐骨神经CCI诱导的上述病理变化，这与普瑞巴林预处理组的减轻效果相似。灰叶斑鸠菊乙醇提取物对坐骨神经CCI诱导的神经性疼痛的改善作用可能归因于黄酮类化合物的存在，并且这种作用归因于这些化合物的抗氧化、神经保护和钙通道调节作用。
