野黄桂精油：体外抗氧化和抗菌活性
Scutia buxifolia Reissek essential oil: in vitro antioxidant and antimicrobial activities.
作者信息
Boligon Aline A, Piana Mariana, Brum Thiele F de, Froeder Amanda L F, Belke Bianca V, Schwanz Thiago G, Mario Débora N, Alves Sydney H, Athayde Margareth L
机构信息
Centro de Ciências da Saúde, Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Núcleo de Análises e Pesquisas Orgânicas/NAPO, Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
出版信息
An Acad Bras Cienc. 2014 Sep;86(3):1463-9. doi: 10.1590/0001-3765201420120034.
The volatile oil from the stem bark of Scutia buxifolia (Rhamnaceae) has been obtained by hydrodistillation and analyzed by GC-MS. Twenty-one components were identified representing 99.93 % of the total oil composition, spathulenol (35.87%), β-cubebene (17.26%), germacrene D (6.43%), linalool (5.19%), carvacrol (4.05%) were the main components of S. buxifolia essential oil. Antioxidant and antimicrobial properties of the essential oil were evaluated by free radical scavenging (DPPH) assay and micro broth dilution method, respectively. S. buxifolia essential oil presented interesting radical scavenging activity (IC50 = 15.03 ± 0.11 µg/mL). The antibacterial assay showed that S. buxifolia stem bark essential oil was moderately active against the Staphylococcus aureus and Micrococcus sp. (MIC = 500 µg/mL) and Escherichia coli (250 µg/mL). To the best of our knowledge, this is the first study on the composition, antioxidant and antimicrobial activities of essential oil from the S. buxifolia collected from Brazil.
通过水蒸馏法从鼠李科铁仔茎皮中提取挥发油，并采用气相色谱 - 质谱联用（GC - MS）进行分析。共鉴定出21种成分，占总油成分的99.93%，其中匙叶桉油烯醇（35.87%）、β - 荜澄茄油烯（17.26%）、杜松烯D（6.43%）、芳樟醇（5.19%）、香芹酚（4.05%）是铁仔精油的主要成分。分别采用自由基清除（DPPH）法和微量肉汤稀释法对精油的抗氧化和抗菌性能进行评价。铁仔精油表现出显著的自由基清除活性（IC50 = 15.03 ± 0.11 µg/mL）。抗菌试验表明，铁仔茎皮精油对金黄色葡萄球菌、微球菌属（MIC = 500 µg/mL）和大肠杆菌（250 µg/mL）具有中等活性。据我们所知，这是首次对采自巴西的铁仔精油的成分、抗氧化和抗菌活性进行研究。
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