评价乳香精油对 T 细胞和树突状细胞的免疫调节作用。
Evaluation of immunomodulatory effects of Boswellia sacra essential oil on T-cells and dendritic cells.
机构信息
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Immunology Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
出版信息
BMC Complement Med Ther. 2020 Nov 19;20(1):352. doi: 10.1186/s12906-020-03146-5.
BACKGROUND
Boswellia sacra resin has been commonly used as analgesic, antimicrobial, and anti-inflammatory properties, which reflect its immunomodulatory activity. Dendritic cells (DCs) are specialized antigen-presenting cells (APCs) and sentinel cells that regulate the immune response. This study aims at investigating whether crude essential oil extracted from Boswellia sacra resin (BSEO), has a potential effect on the phenotype and functions of human monocyte-derived DCs.
METHODS
Oil extract from the resin of Boswellia sacra was prepared by hydrodistillation using a custom made hydrodistiller. BSEO-mediated cell viability has been initially studied on human skin dermis cells (HSD) and DC precursors using quantitative and qualitative assays before applying on DCs. Human DCs were generated from differentiated peripheral blood monocytes cultured in media containing both GM-CSF and IL-4. DCs were exposed to 5 μg/mL or 10 μg/mL of BSEO in vitro. Morphological, phonotypical, and functional properties studied with microscopy, flow cytometry, and ELISA.
RESULTS
Crude BSEO was found to interfere with the maturation and differentiation of DCs from precursor cells in the presence or absence of lipopolysaccharide (LPS). BSEO-treated DCs, cultured in the presence of LPS, reduced the ability of allogeneic T cells to proliferate compared to that co-cultured with LPS-stimulated DCs only. In addition, the endocytic capacity and secretion of IL-10 by DCs treated with BSEO was enhanced in comparison to LPS treated cells. Analysis of the chemical composition of BESO using GC-MS (Clarus 500 GC/MS, PerkinElmer, Shelton, CT) revealed the presence of compounds with several biological activities including antibacterial, antioxidant, and anti-inflammatory properties.
CONCLUSION
Results indicated that BSEO deviates the differentiation of monocytes into immature DCs. Furthermore, stimulation of immature DCs with BSEO was unable to generate full DC maturation. However, these findings may potentially be employed to generate DCs with tolerogenic properties that are able to induce tolerance in diseases with hypersensitivity, autoimmunity as well as transplantation.
背景
乳香树脂常被用作具有镇痛、抗菌和抗炎特性的药物，这反映了其免疫调节活性。树突状细胞（DCs）是专门的抗原提呈细胞（APCs）和哨兵细胞，可调节免疫反应。本研究旨在探讨乳香树脂（BSEO）粗提精油是否对人单核细胞来源的树突状细胞的表型和功能具有潜在影响。
方法
使用定制的水蒸馏器通过水蒸馏法从乳香树脂中提取油提取物。在应用于 DC 之前，通过定量和定性测定法初步研究了油提取物对人皮肤真皮细胞（HSD）和 DC 前体的细胞活力。将外周血单核细胞分化为 DC，在含有 GM-CSF 和 IL-4 的培养基中培养。将 DC 暴露于 5μg/mL 或 10μg/mL 的 BSEO 中进行体外研究。通过显微镜、流式细胞术和 ELISA 研究形态学、表型和功能特性。
结果
发现粗 BSEO 可干扰前体细胞向 DC 的成熟和分化，无论 LPS 是否存在。与仅与 LPS 刺激的 DC 共培养的 DC 相比，在 LPS 存在下培养的 BSEO 处理的 DC 降低了同种异体 T 细胞的增殖能力。此外，与 LPS 处理的细胞相比，BSEO 处理的 DC 的内吞能力和 IL-10 的分泌增强。使用 GC-MS（Clarus 500 GC/MS，PerkinElmer，Shelton，CT）对 BESO 的化学成分进行分析表明，存在具有多种生物学活性的化合物，包括抗菌、抗氧化和抗炎特性。
结论
结果表明，BSEO 使单核细胞分化为未成熟的 DC。此外，用 BSEO 刺激未成熟的 DC 无法产生完全的 DC 成熟。然而，这些发现可能被用来产生具有耐受特性的 DC，这些 DC 能够在具有过敏、自身免疫和移植的疾病中诱导耐受。
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