针对实验性自身免疫性脑脊髓炎的淋巴细胞疫苗接种：疫苗接种方案评估

Lymphocyte vaccination against experimental autoimmune encephalomyelitis: evaluation of vaccination protocols.

作者信息

Offner H, Jones R, Celnik B, Vandenbark A A

机构信息

Neuroimmunology Research, Veterans Administration Medical Center, Portland, OR 97201.

出版信息

J Neuroimmunol. 1989 Jan;21(1):13-22. doi: 10.1016/0165-5728(89)90154-9.

DOI:10.1016/0165-5728(89)90154-9

PMID:2521179

Abstract

Repeated vaccination with encephalitogenic but not other T cell lines could effect marked resistance to 'active' experimental autoimmune encephalomyelitis (EAE) induced by injection of GP-BP in adjuvant. Partial resistance to active EAE was observed in rats recovered from 'passive' line-mediated EAE and in rats vaccinated with T cells attenuated by irradiation or ganglioside treatment. However, no resistance was observed in animals given low doses of activated encephalitogenic T cells. Treatment with hydrostatic pressure alone was found to be ineffective as a means of attenuation, and vaccination with pressure-treated encephalitogenic T cells actually induced mild signs of EAE. However, vaccination with cells that were first pressure treated and then irradiated prevented both clinical and histologic signs of active EAE. In contrast, protection against passive EAE appeared to be clonotypic. Lymphocyte vaccination induced delayed type hypersensitivity (DTH) reactions against autologous T cells, mostly to shared antigens, demonstrating the immunogenicity of multiple antigens on the vaccinating cells.

摘要

用致脑炎性而非其他T细胞系重复接种疫苗，可显著增强对佐剂中注射糖蛋白-结合蛋白（GP-BP）诱导的“主动”实验性自身免疫性脑脊髓炎（EAE）的抵抗力。从“被动”系介导的EAE恢复的大鼠以及接种经辐射或神经节苷脂处理而减毒的T细胞的大鼠，对主动EAE有部分抵抗力。然而，给予低剂量活化的致脑炎性T细胞的动物未观察到抵抗力。单独用流体静压处理作为一种减毒方法无效，接种经压力处理的致脑炎性T细胞实际上会诱发轻度EAE症状。然而，接种先经压力处理然后再经辐射的细胞可预防主动EAE的临床和组织学症状。相比之下，对被动EAE的保护似乎是克隆型的。淋巴细胞接种疫苗诱导针对自体T细胞的迟发型超敏反应（DTH），主要针对共同抗原，表明接种细胞上多种抗原具有免疫原性。