从柚（柑橘属）瓣膜中同时纯化柠檬苦素、诺米林和异obacunoic酸。（注：原文中isoobacunoic acid的“isoobacunoic”可能有误，不太符合常见的化学名称表述，但按照要求准确翻译）

Simultaneous purification of limonin, nomilin and isoobacunoic acid from pomelo fruit (Citrus grandis) segment membrane.

作者信息

Xiang Yu, Cao Jinping, Luo Fenglei, Wang Dengliang, Chen Wei, Li Xian, Sun Chongde, Chen Kunsong

机构信息

Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Univ, Zijingang Campus, Hangzhou, 310058, PR China.

出版信息

J Food Sci. 2014 Oct;79(10):C1956-63. doi: 10.1111/1750-3841.12581. Epub 2014 Sep 11.

DOI:10.1111/1750-3841.12581

PMID:25212475

Abstract

UNLABELLED

A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ-816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi-preparative HPLC. Naringin was partially purified as a by-product of this process using Sephadex LH-20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC-MS/MS.

PRACTICAL APPLICATION

A method of getting health care products limonoids from fruit processing by-products segments membrane of pomelo.

摘要

未标注

建立了一种从柚子（Citrus Grandis）囊衣中同时纯化柠檬苦素、诺米林和异obacunoic酸的方法。该方法包括3个步骤，通过大孔树脂HZ-816去除大部分杂质，用高速逆流色谱法（HSCCC）分离柠檬苦素，并用半制备高效液相色谱法分离诺米林和异obacunoic酸。柚皮苷作为该过程的副产物使用葡聚糖凝胶LH-20进行部分纯化。通过该方法纯化的所有柠檬苦素类化合物纯度达到95%。使用高效液相色谱法根据标准物质的保留时间以及液相色谱-串联质谱法（LC-MS/MS）的特征碎片离子对纯化的柠檬苦素、诺米林和异obacunoic酸进行鉴定。