Panayotov Ivan V, Collart-Dutilleul Pierre-Yves, Salehi Hamideh, Martin Marta, Végh Attila, Yachouh Jacques, Vladimirov Boyan, Sipos Péter, Szalontai Balázs, Gergely Csilla, Cuisinier Frédéric J G
EA4203 Laboratoire de Bio-santé et Nano-science, Université Montpellier 1, Montpellier, France.
Macromol Biosci. 2014 Dec;14(12):1771-82. doi: 10.1002/mabi.201400256. Epub 2014 Sep 11.
Further development of biomaterials is expected as advanced therapeutic products must be compliant to good manufacturing practice regulations. A spraying method for building-up polyelectrolyte films followed by the deposition of dental pulp cells by spraying is presented. Physical treatments of UV irradiation and a drying/wetting process are applied to the system. Structural changes and elasticity modifications of the obtained coatings are revealed by atomic force microscopy and by Raman spectroscopy. This procedure results in thicker, rougher and stiffer film. The initially ordered structure composed of mainly α helices is transformed into random/β-structures. The treatment enhanced dental pulp cell adhesion and proliferation, suggesting that this system is promising for medical applications.
随着先进治疗产品必须符合良好生产规范，生物材料有望得到进一步发展。本文介绍了一种通过喷涂构建聚电解质膜，随后通过喷涂沉积牙髓细胞的方法。对该系统进行了紫外线照射和干燥/湿润过程的物理处理。通过原子力显微镜和拉曼光谱揭示了所得涂层的结构变化和弹性改变。该过程导致形成更厚、更粗糙和更硬的膜。最初主要由α螺旋组成的有序结构转变为无规/β结构。该处理增强了牙髓细胞的粘附和增殖，表明该系统在医学应用方面具有前景。
