Passarini Michel R Z, Miqueletto Paula B, de Oliveira Valéria M, Sette Lara D
Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas-CPQBA, Universidade Estadual de Campinas-UNICAMP, Rua Alexandre Cazelatto, Paulínia, SP, Brazil.
J Basic Microbiol. 2015 Feb;55(2):207-20. doi: 10.1002/jobm.201400466. Epub 2014 Sep 11.
The present work aimed to investigate the diversity of bacteria and filamentous fungi of southern Atlantic Ocean marine sponge Dragmacidon reticulatum using cultivation-independent approaches. Fungal ITS rDNA and 18S gene analyses (DGGE and direct sequencing approaches) showed the presence of representatives of three order (Polyporales, Malasseziales, and Agaricales) from the phylum Basidiomycota and seven orders belonging to the phylum Ascomycota (Arthoniales, Capnodiales, Dothideales, Eurotiales, Hypocreales, Pleosporales, and Saccharomycetales). On the other hand, bacterial 16S rDNA gene analyses by direct sequencing approach revealed the presence of representatives of seven bacterial phyla (Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Lentisphaerae, Chloroflexi, and Planctomycetes). Results from statistical analyses (rarefaction curves) suggested that the sampled clones covered the fungal diversity in the sponge samples studied, while for the bacterial community additional sampling would be necessary for saturation. This is the first report related to the molecular analyses of fungal and bacterial communities by cultivation-independent approaches in the marine sponges D. reticulatum. Additionally, the present work broadening the knowledge of microbial diversity associated to marine sponges and reports innovative data on the presence of some fungal genera in marine samples.
本研究旨在采用非培养方法调查南大西洋海洋海绵网状韧海绵（Dragmacidon reticulatum）中细菌和丝状真菌的多样性。真菌ITS rDNA和18S基因分析（变性梯度凝胶电泳和直接测序方法）表明，存在来自担子菌门的三个目（多孔菌目、马拉色菌目和伞菌目）的代表以及属于子囊菌门的七个目（节担菌目、多隔孢菌目、座囊菌目、散囊菌目、肉座菌目、格孢腔菌目和酵母目）的代表。另一方面，通过直接测序方法对细菌16S rDNA基因进行分析，结果显示存在七个细菌门（蓝细菌门、变形菌门、放线菌门、拟杆菌门、浮霉菌门、绿弯菌门和浮霉菌门）的代表。统计分析（稀疏曲线）结果表明，所采样的克隆涵盖了所研究海绵样品中的真菌多样性，而对于细菌群落，要达到饱和则需要额外采样。这是首次有关采用非培养方法对网状韧海绵中真菌和细菌群落进行分子分析的报告。此外，本研究拓宽了与海洋海绵相关的微生物多样性知识，并报告了海洋样品中一些真菌属存在情况的创新数据。
