Sun Shangde, Zhou Wenya
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology.
J Oleo Sci. 2014;63(10):1011-8. doi: 10.5650/jos.ess14095. Epub 2014 Sep 10.
Feruloylated lipids are esters of ferulic acid, which have antioxidation and UV-absorbing activities. In this study, lipophilic feruloylated lipids were prepared by the enzymatic transesterification of ethyl ferulate (EF) with distearin. Effects of different diacylglycerols as feruloyl acceptors were compared. Effects of transesterification variables were studied and optimized using response surface methodology. Under the optimized conditions (reaction temperature 78°C, reaction time 24 h, and enzyme load 14%), EF conversion was 97.6±2.2%, and the lipophilic feruloylated lipids prepared by the transesterification were consisted of 43.2±0.9% feruloylated monoacylglycerols (FMAG) and 33.3±1.3% feruloylated diacylglycerols (FDAG), respectively. Analysis of variance (ANOVA) showed that the regression equation was adequate for predicting the transesterification. The activation energies for the transesterification of EF with distearin to form lipophilic FMAG + FDAG and the side reaction hydrolysis of EF to form glyceryl ferulate + glyceryl diferulate were 57.75 and 40.31 kJ/mol, respectively.
阿魏酸酯化脂质是阿魏酸的酯类，具有抗氧化和紫外线吸收活性。在本研究中，通过阿魏酸乙酯（EF）与二硬脂酸甘油酯的酶促酯交换反应制备了亲脂性阿魏酸酯化脂质。比较了不同二酰基甘油作为阿魏酰受体的效果。使用响应面法研究并优化了酯交换反应变量。在优化条件下（反应温度78°C，反应时间24小时，酶负载量14%），EF转化率为97.6±2.2%，通过酯交换反应制备的亲脂性阿魏酸酯化脂质分别由43.2±0.9%的阿魏酰化单酰基甘油（FMAG）和33.3±1.3%的阿魏酰化二酰基甘油（FDAG）组成。方差分析（ANOVA）表明回归方程足以预测酯交换反应。EF与二硬脂酸甘油酯酯交换形成亲脂性FMAG + FDAG的活化能以及EF水解形成阿魏酸甘油酯+二阿魏酸甘油酯的副反应活化能分别为57.75和40.31 kJ/mol。
