Zheng Yan, Wu Xiao-Mei, Branford-White Christopher, Quan Jing, Zhu Li-Min
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, PR China.
Bioresour Technol. 2009 Jun;100(12):2896-901. doi: 10.1016/j.biortech.2009.01.042. Epub 2009 Feb 28.
Feruloylated diacylglycerol (FDAG) was synthesized using a selective lipase-catalyzed the transesterification between ethyl ferulate and triolein. To optimize the reaction conversion and purity of FDAG, dual response surface was applied to determine the effects of five-level-five-factors and their reciprocal interactions on product synthesis. A total of 32 individual experiments were performed to study reaction temperature, reaction time, substrate molar ratio, enzyme loading, and water activity. The highest reaction conversion and selectivity towards FDAG were 73.9% and 92.3%, respectively, at 55 degrees C, reaction time 5.3 day, enzyme loading 30.4 mg/ml, water activity 0.08, and a substrate molar ratio of 3.7. Moreover, predicted values showed good validation with the experimental values when experiments corresponding to selected points on the contour plots were carried out.
阿魏酸二酰甘油（FDAG）是通过选择性脂肪酶催化阿魏酸乙酯与三油酸甘油酯之间的酯交换反应合成的。为了优化FDAG的反应转化率和纯度，采用双响应面法来确定五水平五因素及其相互作用对产物合成的影响。总共进行了32次独立实验，以研究反应温度、反应时间、底物摩尔比、酶负载量和水分活度。在55℃、反应时间5.3天、酶负载量30.4mg/ml、水分活度0.08和底物摩尔比3.7的条件下，最高反应转化率和对FDAG的选择性分别为73.9%和92.3%。此外，当进行与等高线图上选定点对应的实验时，预测值与实验值显示出良好的吻合度。
