3-氨基苯甲酰胺对唐氏综合征淋巴细胞中X射线诱导染色体畸变的影响。

The effect of 3-aminobenzamide on X-ray induction of chromosome aberrations in Down syndrome lymphocytes.

作者信息

MacLaren R A, Au W W, Legator M S

机构信息

University of Texas Medical Branch, Department of Preventive Medicine and Community Health, Galveston 77550.

出版信息

Mutat Res. 1989 Jan;222(1):1-7. doi: 10.1016/0165-1218(89)90029-3.

DOI:10.1016/0165-1218(89)90029-3

PMID:2521372

Abstract

Human lymphocytes from normal and Down syndrome (DS) subjects were examined to determine the effect of 3-aminobenzamide (3AB) on X-ray-induced chromosome aberrations. Lymphocytes were treated with 150 or 300 rad of X-rays in the presence of 3 mM 3AB for various times after irradiation, and then the cells were analyzed for the presence of chromosome aberrations in mitotic cells. 3-Aminobenzamide had no effect on the frequency of chromosome aberrations produced by X-rays in G0 lymphocytes from normal subjects. In contrast, lymphocytes from DS patients displayed an increase in the frequency of chromosome aberrations as a result of treatment with X-rays in the presence of 3AB. These observations indicate that DS lymphocytes are more sensitive to the inhibition of poly(ADP)ribose synthetase than normal lymphocytes.

摘要

对来自正常人和唐氏综合征（DS）患者的人类淋巴细胞进行检测，以确定3-氨基苯甲酰胺（3AB）对X射线诱导的染色体畸变的影响。淋巴细胞在照射后不同时间，于3 mM 3AB存在的情况下接受150或300拉德的X射线照射，然后分析有丝分裂细胞中染色体畸变的情况。3-氨基苯甲酰胺对正常受试者G0期淋巴细胞中X射线产生的染色体畸变频率没有影响。相比之下，在3AB存在的情况下，DS患者的淋巴细胞经X射线处理后，染色体畸变频率增加。这些观察结果表明，DS淋巴细胞比正常淋巴细胞对聚（ADP）核糖合成酶的抑制更敏感。