大鼠心房利钠因子（ANP-III）抑制来自浅表和近髓质皮质的刷状缘膜中的磷酸盐转运。

Rat atrial natriuretic factor (ANP-III) inhibits phosphate transport in brush border membrane from superficial and juxtamedullary cortex.

作者信息

Yusufi A N, Berndt T J, Moltaji H, Donovan V, Dousa T P, Knox F G

机构信息

Department of Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55905.

出版信息

Proc Soc Exp Biol Med. 1989 Jan;190(1):87-90. doi: 10.3181/00379727-190-42833.

DOI:10.3181/00379727-190-42833

PMID:2521395

Abstract

Previous studies have shown that administration of synthetic atrial natriuretic factor (ANF, 101-126) decreases sodium-dependent phosphate transport across renal brush border membrane vesicles (BBMV) in rats fed a normal or low phosphate diet. In the present study, infusion of rat ANF (atriopeptin III (ANP-III), 103-126 rat ANF) to rats fed a normal phosphate diet caused natriuretic and phosphaturic effects similar to those of ANF (101-126), but unlike ANF (101-126) did not increase the glomerular filtration rate. The effect of ANP-III infusion on sodium-dependent transport of phosphate was also determined in BBM vesicles isolated from the superficial cortex (BBMV-SC) and juxtamedullary cortex (BBMV-JM). The results indicate that ANP-III decreases phosphate transport across BBMV-SC and BBMV-JM similarly (20-24%). However, it had no effect on sodium-dependent transport of proline in these vesicles. The infusion of ANP-III to rats fed a normal phosphate diet inhibits phosphate uptake both in BBMV-SC and BBMV-JM and causes phosphaturia without increments in glomerular filtration rate.

摘要

先前的研究表明，给予合成心房利钠因子（ANF，101 - 126）可降低正常或低磷饮食喂养的大鼠肾刷状缘膜囊泡（BBMV）中钠依赖性磷酸盐转运。在本研究中，向正常磷饮食喂养的大鼠输注大鼠ANF（心房肽III（ANP - III），103 - 126大鼠ANF）产生的利钠和利磷作用与ANF（101 - 126）相似，但与ANF（101 - 126）不同的是，它并未增加肾小球滤过率。还在从浅表皮质（BBMV - SC）和近髓皮质（BBMV - JM）分离的BBM囊泡中测定了ANP - III输注对钠依赖性磷酸盐转运的影响。结果表明，ANP - III同样程度地降低了跨BBMV - SC和BBMV - JM的磷酸盐转运（20 - 24%）。然而，它对这些囊泡中脯氨酸的钠依赖性转运没有影响。向正常磷饮食喂养的大鼠输注ANP - III可抑制BBMV - SC和BBMV - JM中的磷酸盐摄取，并导致利磷作用，而肾小球滤过率无增加。