真武汤颗粒对压力超负荷大鼠左心室心肌肥厚的影响。

Effect of Zhenwu Tang Granule on pressure-overloaded left ventricular myocardial hypertrophy in rats.

机构信息

Department of Emergency Medicine, Red Cross Hospital of Guangzhou, Guangzhou 510220,China (Xie ZX); Department of Emergency Medicine. First Hospital Affiliated to Guangzhou Medical University, Guangzhou 510120, China (Wang SY, Liang ZJ, Zeng LB).

出版信息

World J Emerg Med. 2010;1(2):149-53.

PMID:25214959

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4129748/

Abstract

BACKGROUND

In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect of Zhenwu Tang Granule on chronic pressure-overloaded left ventricular hypertrophy in rats.

METHODS

The study was performed at the laboratory of Guangzhou Institute of Respiratory Disease. Male SD rats were divided randomly into 3 groups: sham operation group (n=8), operation group (n=15) and traditional Chinese medicine (TCM) group (n=15). The model of myocardial hypertrophy was made by gradually constricting the abdominal aorta. Sixteen weeks later, cardiac ultrasonography was performed in all groups in order to ascertain post-operational left ventricular (LV) hypertrophy. And Zhenwu Tang Granule was added at a dose of 12 g/kg in the mixed feedstuff for 8 weeks in the TCM group. In the 24th week, weight, structure as well as function of the heart in each group were measured by high-frequency ultrasonography, and Masson's staining was performed on the cardiac muscles. Meanwhile, total collagen volume fraction (CVF-T) and non-coronary vessel collagen volume fraction (CVF-NV) were analyzed.

RESULTS

There was an increase in the weight of the heart in the operation group, with the left ventricule dominated (P<0.05). The heart was enlarged, with diastolic interventricular septal distance (IVSd) and left ventricular posterior wall distance (LVPWd) dominated (P<0.01). There was a significant decrease in the cardiac function (P<0.05). The weight (P<0.01) and volume of the heart decreased in the TCM group compared with the operation group, with IVSd and systolic left ventricular posterior wall dominated (P<0.01). And the cardiac function was improved (P<0.05). Significant interstitial and collagen hyperplasia was shown in the operation group based on pathological analysis, and various improvements were proved in the TCM group, i.e. there was a significant decrease in CVF-T and CVF-NV (P<0.01) compared with the operation group; but no difference (P>0.05) was found when compared with the pseudo-operation group.

CONCLUSION

Zhenwu Tang Granule could reduce the weight and volume of the heart, improve the cardiac function, inhibit hyperplasia of collagen, and reverse myocardial hypertrophy in rats with pressure-overloaded left ventricular hypertrophy.

摘要

背景

在中医视角下，尽管已有很多研究，但对复方制剂的关注较少。本研究旨在探讨真武汤颗粒对大鼠慢性压力超负荷左心室肥厚的影响。

方法

该研究在广州呼吸疾病研究所的实验室进行。雄性 SD 大鼠随机分为 3 组：假手术组（n=8）、手术组（n=15）和中药组（n=15）。通过逐渐缩窄腹主动脉来建立心肌肥厚模型。16 周后，所有组均进行心脏超声检查以确定术后左心室（LV）肥厚。中药组同时在混合饲料中添加真武汤颗粒，剂量为 12 g/kg，连续 8 周。在第 24 周，通过高频超声测量各组的体重、心脏结构和功能，并对心肌进行 Masson 染色。同时，分析总胶原容积分数（CVF-T）和非冠状动脉血管胶原容积分数（CVF-NV）。

结果

手术组的心脏重量增加，以左心室为主（P<0.05）。心脏增大，以舒张室间隔距离（IVSd）和左心室后壁距离（LVPWd）为主（P<0.01）。心功能明显下降（P<0.05）。与手术组相比，中药组的心脏重量（P<0.01）和体积减小，以 IVSd 和收缩左心室后壁为主（P<0.01），心功能得到改善（P<0.05）。病理学分析显示手术组有明显的间质和胶原增生，中药组则有各种改善，即 CVF-T 和 CVF-NV 显著降低（P<0.01），与手术组相比；但与假手术组相比无差异（P>0.05）。