Huang Juan, Marchal Elisabeth, Hult Ekaterina F, Zels Sven, Vanden Broeck Jozef, Tobe Stephen S
Department of Cell and Systems Biology, University of Toronto, Toronto, Canada.
Department of Cell and Systems Biology, University of Toronto, Toronto, Canada; Department of Biology, Zoological Institute, KU Leuven, B-3000 Leuven, Belgium.
Insect Biochem Mol Biol. 2014 Nov;54:61-8. doi: 10.1016/j.ibmb.2014.09.001. Epub 2014 Sep 10.
The FGLamide allatostatins (FGL/ASTs) are a family of neuropeptides with pleiotropic functions, including the inhibition of juvenile hormone (JH) biosynthesis, vitellogenesis and muscle contraction. In the cockroach, Diploptera punctata, thirteen FGLa/ASTs and one allatostatin receptor (AstR) have been identified. However, the mode of action of ASTs in regulation of JH biosynthesis remains unclear. Here, we determined the tissue distribution of Dippu-AstR. And we expressed Dippu-AstR in vertebrate cell lines, and activated the receptor with the Dippu-ASTs. Our results show that all thirteen ASTs activated Dippu-AstR in a dose dependent manner, albeit with different potencies. Functional analysis of AstR in multiple cell lines demonstrated that activation of the AstR receptor resulted in elevated levels of Ca(2+) and cAMP, which suggests that Dippu-AstR can act through the Gαq and Gαs protein pathways. The study on the target of AST action reveals that FGL/AST affects JH biosynthesis prior to the entry of acetyl-CoA into the JH biosynthetic pathway.
FGL酰胺类咽侧体静止素（FGL/ASTs）是一类具有多种功能的神经肽，包括抑制保幼激素（JH）生物合成、卵黄生成和肌肉收缩。在蟑螂双斑大蠊中，已鉴定出13种FGLa/ASTs和一种咽侧体静止素受体（AstR）。然而，ASTs在调节JH生物合成中的作用模式仍不清楚。在此，我们确定了双斑大蠊AstR（Dippu-AstR）的组织分布。我们在脊椎动物细胞系中表达了Dippu-AstR，并用双斑大蠊ASTs激活该受体。我们的结果表明，所有13种ASTs均以剂量依赖方式激活Dippu-AstR，尽管其效力不同。在多个细胞系中对AstR进行功能分析表明，AstR受体的激活导致Ca(2+)和cAMP水平升高，这表明Dippu-AstR可通过Gαq和Gαs蛋白途径发挥作用。对AST作用靶点的研究表明，FGL/AST在乙酰辅酶A进入JH生物合成途径之前影响JH生物合成。
