通过聚合酶链式反应-变性梯度凝胶电泳快速鉴定蜜环菌属菌种
Rapid identification of Armillaria species by PCR-DGGE.
作者信息
Longa Claudia M O, La Porta Nicola
机构信息
Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.
Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.
出版信息
J Microbiol Methods. 2014 Dec;107:63-5. doi: 10.1016/j.mimet.2014.09.002. Epub 2014 Sep 16.
The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armillaria species in Europe.
由于利用形态特征时存在模糊性，分子方法已被用于区分蜜环菌物种。使用真菌引物ITS3GC-ITS4的PCR-DGGE能够选择性分离扩增子，并提供一种简单快速的方法，一步鉴别出欧洲最常见的蜜环菌物种。
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