Suppr超能文献

通过聚合酶链式反应-变性梯度凝胶电泳快速鉴定蜜环菌属菌种

Rapid identification of Armillaria species by PCR-DGGE.

作者信息

Longa Claudia M O, La Porta Nicola

机构信息

Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.

Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.

出版信息

J Microbiol Methods. 2014 Dec;107:63-5. doi: 10.1016/j.mimet.2014.09.002. Epub 2014 Sep 16.

DOI:10.1016/j.mimet.2014.09.002
PMID:25218110
Abstract

The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armillaria species in Europe.

摘要

由于利用形态特征时存在模糊性，分子方法已被用于区分蜜环菌物种。使用真菌引物ITS3GC-ITS4的PCR-DGGE能够选择性分离扩增子，并提供一种简单快速的方法，一步鉴别出欧洲最常见的蜜环菌物种。

相似文献

1
Rapid identification of Armillaria species by PCR-DGGE.
J Microbiol Methods. 2014 Dec;107:63-5. doi: 10.1016/j.mimet.2014.09.002. Epub 2014 Sep 16.
2
A new multilocus approach for a reliable DNA-based identification of Armillaria species.
Mycologia. 2013 Jul-Aug;105(4):1059-76. doi: 10.3852/12-209. Epub 2013 Feb 28.
3
The rapid identification of European Armillaria species from soil samples by nested PCR.
FEMS Microbiol Lett. 2004 Aug 1;237(1):105-10. doi: 10.1016/j.femsle.2004.06.019.
4
Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene.
Mycologia. 2010 Jul-Aug;102(4):898-910. doi: 10.3852/09-238.
5
Characterization of Cladosporium Species by Internal Transcribed Spacer-PCR and Microsatellites-PCR.
Pak J Biol Sci. 2016;19(4):143-157. doi: 10.3923/pjbs.2016.143.157.
6
A rapid infection assay for Armillaria and real-time PCR quantitation of the fungal biomass in planta.
Fungal Biol. 2010 Jan;114(1):107-19. doi: 10.1016/j.mycres.2009.11.003. Epub 2009 Nov 11.
7
Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting.
Res Microbiol. 2007 Mar;158(2):112-21. doi: 10.1016/j.resmic.2006.10.003. Epub 2006 Dec 20.
8
Evaluation of partial tef1, rpb2, and nLSU sequences for identification of isolates representing Armillaria calvescens and Armillaria gallica from northeastern North America.
Fungal Biol. 2011 Aug;115(8):741-9. doi: 10.1016/j.funbio.2011.05.008. Epub 2011 May 30.
9
Amplification of soil fungal community DNA using the ITS86F and ITS4 primers.
FEMS Microbiol Lett. 2009 Jul;296(1):91-6. doi: 10.1111/j.1574-6968.2009.01621.x. Epub 2009 May 11.
10
Armillaria altimontana, a new species from the western interior of North America.
Mycologia. 2012 Sep-Oct;104(5):1200-5. doi: 10.3852/11-409. Epub 2012 Apr 13.

引用本文的文献

1
Epidemiology, Biotic Interactions and Biological Control of Armillarioids in the Northern Hemisphere.
Pathogens. 2021 Jan 16;10(1):76. doi: 10.3390/pathogens10010076.
2
Evaluation of the Bacterial Diversity of Inner Mongolian Acidic Gruel Using Illumina MiSeq and PCR-DGGE.
Curr Microbiol. 2020 Mar;77(3):434-442. doi: 10.1007/s00284-019-01848-9. Epub 2020 Jan 1.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验