Wang Tengfei, Li Yuxiang, Zhao Peng, Wang Jie, Zhang Xiaomin, Hao Yinju, Du Juan, Zhao Chengjun, Sun Tao, Yu Jianqiang, Zhou Ru, Jin Shaoju
Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, PR China.
College of Nursing, Ningxia Medical University, Yinchuan 750004, PR China.
Ann Indian Acad Neurol. 2014 Jul;17(3):313-6. doi: 10.4103/0972-2327.138513.
Our previous studies demonstrated that oxysophoridine (OSR) had neuroprotective effects on mice through antioxidant and anti-apoptotic mechanisms. In this study, we investigated whether OSR could influence the release of amino acids in ischemic mice brains.
Male ICR mice were scheduled to undergo 2 h middle cerebral artery occlusion (MCAO) and 24 h reperfusion. Before MCAO, mice in corresponding groups were intraperitoneally injected with OSR (62.5, 125 and 250 mg/kg) for seven successive days. After reperfusion, neurological scores were estimated, infarct volume and the brain water content were assessed. The levels of glutamate (Glu), aspartate (Asp), γ-aminobutyric acid (GABA) and Glycine (Gly) were measured by amino acid analyzer.
OSR significantly decreased neurological scores, reduced infarct volume and the brain water content. After treatment with OSR of 250 mg/kg, the contents of Glu, Asp, GABA and Gly in mice brains could maintain at a normal level compared with MCAO group mice. The Glu/GABA ratio was significantly decreased in OSR group mice.
These findings indicate that OSR has a protective effect on cerebral ischemic injury and helps to maintain the amino acids homeostasis after reperfusion for a long time.
我们先前的研究表明氧化苦参碱（OSR）通过抗氧化和抗凋亡机制对小鼠具有神经保护作用。在本研究中，我们调查了OSR是否会影响缺血小鼠脑内氨基酸的释放。
雄性ICR小鼠接受2小时大脑中动脉闭塞（MCAO）并再灌注24小时。在MCAO之前，相应组的小鼠连续7天腹腔注射OSR（62.5、125和250mg/kg）。再灌注后，评估神经功能评分、梗死体积和脑含水量。通过氨基酸分析仪测量谷氨酸（Glu）、天冬氨酸（Asp）、γ-氨基丁酸（GABA）和甘氨酸（Gly）的水平。
OSR显著降低神经功能评分，减少梗死体积和脑含水量。用250mg/kg的OSR处理后，与MCAO组小鼠相比，小鼠脑内Glu、Asp、GABA和Gly的含量可维持在正常水平。OSR组小鼠的Glu/GABA比值显著降低。
这些发现表明OSR对脑缺血损伤具有保护作用，并有助于在再灌注后长时间维持氨基酸稳态。
