Ma X X, Feng Y P, Liu J L, Chen L, Zhao Y Q, Guo P H, Guo J Z, Ma L Y, Ma Z R
Key Laboratory of Bioengineering & Biotechnology of State Ethnic Affairs Commission, Engineering & Technology Research Center for Animal Cell, College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou, China.
Key Laboratory of Bioengineering & Biotechnology of State Ethnic Affairs Commission, Engineering & Technology Research Center for Animal Cell, College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou, China
Genet Mol Res. 2014 Sep 5;13(3):7347-55. doi: 10.4238/2014.September.5.13.
Adaptation in the overall codon usage pattern of West Nile virus (WNV) to that of two hosts was estimated based on the synonymous codon usage value (RSCU). Synonymous codon usage biases for the beginning coding sequence of this virus were also analyzed by calculating the usage fluctuation for each synonymous codon along the target region (the first 270 codon sites of the whole coding sequence of WNV). Adaptation of WNV to Anopheles gambiae regarding the overall codon usage revealed a mixture of synonymous codon usage patterns between this virus and its vector. Regarding the adaptation of WNV to its dead-end host and codon usage, although a mixture of overall codon usage patterns exists, the number of codons with reversed tendency codon usage is lower than that between the virus and its vector. In addition, some codons with low RSCU values for this virus are highly selected in the beginning translation region of WNV, while codons with low RSCU values in this region tend to pair with tRNAs present in low abundance in the host, suggesting that highly selected codons in a specific region in the beginning region of WNV are, to some degree, influenced by the corresponding low tRNA abundance of hosts to regulate the translation speed of the WNV polyprotein.
基于同义密码子使用值（RSCU）估算了西尼罗河病毒（WNV）整体密码子使用模式对两种宿主的适应性。还通过计算每个同义密码子沿目标区域（WNV整个编码序列的前270个密码子位点）的使用波动，分析了该病毒起始编码序列的同义密码子使用偏好。WNV在整体密码子使用方面对冈比亚按蚊的适应性揭示了该病毒与其载体之间同义密码子使用模式的混合情况。关于WNV对其终末宿主的适应性和密码子使用情况，尽管存在整体密码子使用模式的混合，但密码子使用倾向逆转的密码子数量低于病毒与其载体之间的情况。此外，该病毒中一些RSCU值较低的密码子在WNV的起始翻译区域中被高度选择，而该区域中RSCU值较低的密码子往往与宿主中丰度较低的tRNA配对，这表明WNV起始区域特定区域中高度选择的密码子在一定程度上受宿主相应低tRNA丰度的影响，以调节WNV多聚蛋白的翻译速度。
