采用氟-18-脱氧葡萄糖正电子发射断层扫描/计算机断层扫描联合Ki67和葡萄糖转运蛋白1免疫组织化学评估齐墩果酸对C6大鼠胶质瘤的放射增敏作用

Fluorine-18-deoxyglucose positron emission tomography/computed tomography with Ki67 and GLUT-1 immunohistochemistry for evaluation of the radiosensitization effect of oleanolic acid on C6 rat gliomas.

作者信息

Zhang Yu, Xu Huiqin, Wang Hui, Yu Wenjing, Zhao Xuefeng, Xue Yangyang

机构信息

Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

出版信息

Nucl Med Commun. 2015 Jan;36(1):21-7. doi: 10.1097/MNM.0000000000000211.

DOI:10.1097/MNM.0000000000000211

PMID:25222909

Abstract

OBJECTIVE

The aim of this study was to investigate the radiosensitization effect of oleanolic acid (OA) in an in-vivo C6 rat glioma model using fluorine-18-deoxyglucose PET/computed tomography (18F-FDG PET/CT) and Ki67 and glucose transporter-1 (GLUT-1) immunohistochemistry(IHC) and evaluate the utility of 18F-FDG PET/CT in assessing early changes after radiotherapy.

MATERIALS AND METHODS

Tumor-bearing rats were divided into four groups: the control group (group A), the OA group(group B), the radiotherapy group (group C), and the OA combined with radiotherapy group (group D). 18F-FDG PET/CT images were obtained to monitor the tumor/muscle (T/M) ratio of 18F-FDG uptake before treatment,1 day after treatment, and 7 days after treatment. Tumor volume changes were also assessed, and hematoxylin and eosin staining and Ki67 and GLUT-1 IHC staining were also carried out.

RESULTS

Before treatment, there were no obvious differences between the T/M ratios (F=0.147, P=0.931)and tumor volumes (F=0.177, P=0.911) among the four groups. At day 1 after treatment, statistical differences were observed in the T/M ratios (F=2.891, P=0.05), with decreased values in groups C and D compared with group A(tCA=2.354, tDA=2.356, P<0.05). At day 7 after treatment,the T/M values of group D were lower than those of the other three groups (tDC=2.416, P=0.03; tDA=8.133,tDB=7.656, P<0.01), and the tumor volumes of group D were significantly decreased compared with group C(t=2.351, P=0.02). There were significant differences in tumor cell densities and in the expression of Ki67 and GLUT-1 in the four groups (F=16.667, 22.082, and 39.555,P<0.001). The T/M ratio showed a significant correlation with the expression of Ki67 and GLUT-1, and our study also found a significant relationship between the expression of Ki67 and the expression of GLUT-1.

CONCLUSION

OA has a radiosensitization effect on C6 rat glioma tumors in vivo, detected using 18F-FDG PET/CT and Ki67 and GLUT-1 IHC staining. 18F-FDG PET/CT is a potentially sensitive tool for the evaluation of early changes after radiotherapy.

摘要

目的

本研究旨在使用氟 - 18 - 脱氧葡萄糖正电子发射断层扫描/计算机断层扫描（18F - FDG PET/CT）以及Ki67和葡萄糖转运蛋白 - 1（GLUT - 1）免疫组织化学（IHC），研究齐墩果酸（OA）在体内C6大鼠胶质瘤模型中的放射增敏作用，并评估18F - FDG PET/CT在评估放疗后早期变化中的效用。

材料与方法

荷瘤大鼠分为四组：对照组（A组）、OA组（B组）、放疗组（C组）和OA联合放疗组（D组）。在治疗前、治疗后1天和治疗后7天获取18F - FDG PET/CT图像，以监测18F - FDG摄取的肿瘤/肌肉（T/M）比值。还评估了肿瘤体积变化，并进行苏木精和伊红染色以及Ki67和GLUT - 1 IHC染色。

结果

治疗前，四组之间的T/M比值（F = 0.147，P = 0.931）和肿瘤体积（F = 0.177，P = 0.911）无明显差异。治疗后1天，T/M比值存在统计学差异（F = 2.891，P = 0.05），C组和D组的值低于A组（tCA = 2.354，tDA = 2.356，P < 0.05）。治疗后7天，D组的T/M值低于其他三组（tDC = 2.416，P = 0.03；tDA = 8.133，tDB = 7.656，P < 0.01），且D组的肿瘤体积与C组相比显著减小（t = 2.351，P = 0.02）。四组肿瘤细胞密度以及Ki67和GLUT - 1的表达存在显著差异（F = 16.667、22.082和39.555，P < 0.001）。T/M比值与Ki67和GLUT - 1的表达呈显著相关，并且我们的研究还发现Ki67的表达与GLUT - 1的表达之间存在显著关系。