Katada Ryuichi, Sugimoto Kana, Yoshida Motonori, Matsumoto Hiroshi
Nihon Arukoru Yakubutsu Igakkai Zasshi. 2014 Jun;49(3):188-94.
Ethanol increases brain aquaporin-4 (AQP4) expression after traumatic brain injury (TBI), leading to augment mortality and morbidity after TBI. AQP4 is regulated by sodium ion channels/transporters. Ethanol affects the ion channels/transporters. From these findings, we hypothesized that ethanol may have different effects on AQP4 expression in hypo- or hyper-sodium condition. In this study, rat primary astrocytes were incubated in iso-, hypo- or hyper-sodium MEM medium with 10% calf serum. Ethanol was added to each medium simultaneously. And to check whether hypo/hyper-sodium condition could change AQP4 expression after ethanol exposure or not, astrocytes were incubated in iso-sodium with ethanol, followed by changed to hypo/hyper-sodium with the same concentration of ethanol. Astrocyte AQP4 expression was increased in hypo-sodium exposure. Hypo-sodium with ethanol did not change AQP4 expression significantly, on the other hand, hyper-sodium with ethanol decreased AQP4 expression for short time exposure, and increased it for long time exposure. Hyper-sodium changing increased astrocyte AQP4 expression under EtOH exposure. These findings suggest that AQP4 expression is regulated by sodium ion or ion channels/transporters. And ethanol affects sodium ion channels/transporters, which is involved in AQP4 expression under ethanol.
乙醇会增加创伤性脑损伤（TBI）后脑水通道蛋白-4（AQP4）的表达，导致TBI后死亡率和发病率上升。AQP4受钠离子通道/转运体调控。乙醇会影响离子通道/转运体。基于这些发现，我们推测乙醇在低钠或高钠状态下可能对AQP4表达有不同影响。在本研究中，将大鼠原代星形胶质细胞置于含10%小牛血清的等渗、低渗或高渗MEM培养基中培养。同时向每种培养基中加入乙醇。为了检测低渗/高渗状态是否会在乙醇暴露后改变AQP4表达，先将星形胶质细胞置于含乙醇的等渗培养基中培养，然后更换为含相同浓度乙醇的低渗/高渗培养基。低钠暴露会增加星形胶质细胞AQP4表达。低钠加乙醇对AQP4表达无显著改变，另一方面，高钠加乙醇在短时间暴露时会降低AQP4表达，长时间暴露则会增加。在乙醇暴露下，高钠变化会增加星形胶质细胞AQP4表达。这些发现表明AQP4表达受钠离子或离子通道/转运体调控。并且乙醇会影响钠离子通道/转运体，这与乙醇作用下的AQP4表达有关。
