Mukuda Takao, Koyama Yuka, Hamasaki Sawako, Kaidoh Toshiyuki, Furukawa Yasuo
Laboratory of Neurobiology, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-hiroshima, Japan; Department of Anatomy, Faculty of Medicine, Tottori University, Yonago, Japan.
Laboratory of Neurobiology, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-hiroshima, Japan; Department of Anatomy, Faculty of Medicine, Tottori University, Yonago, Japan.
Brain Res. 2014 Nov 7;1588:92-103. doi: 10.1016/j.brainres.2014.09.019. Epub 2014 Sep 16.
Physical exercise is a robust stimulus that enhances hippocampal neurogenesis via cell proliferation in rodents. We examined the role of systemic angiotensin (Ang) peptides in exercise-dependent enhancement of neurogenesis in the adult rat hippocampus. Plasma angiotensin peptide concentration increased rapidly in response to 30 min of treadmill exercise. After undertaking this exercise once daily for a week, the number of proliferating cells in the hippocampus, identified by 5-bromo-2'-deoxyuridine (BrdU) incorporation, had increased compared with controls. To mimic the increase in plasma Ang peptide concentrations brought about by exercise, rats were injected with 10(-5)M Ang II once daily for a week. The number of BrdU-incorporating cells and of doublecortin (DCX)-expressing immature neurons in the hippocampus rose approximately 1.5 and 1.9-fold compared with controls, respectively. The effects were completely abolished by an Ang II receptor subtype 1 antagonist losartan. These findings, taken together, suggest that an increased concentrations of Ang peptides in the systemic circulation during exercise may promote neurogenesis in the adult rat hippocampus.
体育锻炼是一种强大的刺激因素，可通过促进啮齿动物的细胞增殖来增强海马体神经发生。我们研究了全身血管紧张素（Ang）肽在成年大鼠海马体中运动依赖性神经发生增强过程中的作用。跑步机运动30分钟后，血浆血管紧张素肽浓度迅速升高。在每天进行一次这种运动，持续一周后，通过5-溴-2'-脱氧尿苷（BrdU）掺入法鉴定的海马体中增殖细胞的数量与对照组相比有所增加。为了模拟运动引起的血浆Ang肽浓度升高，大鼠每天注射一次10(-5)M的Ang II，持续一周。与对照组相比，海马体中掺入BrdU的细胞数量和表达双皮质素（DCX）的未成熟神经元数量分别增加了约1.5倍和1.9倍。血管紧张素II受体1型拮抗剂氯沙坦完全消除了这些作用。综上所述，这些发现表明运动期间全身循环中Ang肽浓度的升高可能促进成年大鼠海马体中的神经发生。
