Fidel P L, Murphy J W
Department of Botany and Microbiology, University of Oklahoma, Norman 73019.
Infect Immun. 1989 Apr;57(4):1158-64. doi: 10.1128/iai.57.4.1158-1164.1989.
Cyclosporin A (CsA), a potent immunosuppressive drug, was used to explore further the induction, expression, and regulation of lymphoid cells involved in the delayed-type hypersensitivity (DTH) response to cryptococcal antigen(s). We found that the induction of the cells responsible for DTH (TDH cells) was not affected by CsA, but their expression was inhibited in CsA-treated mice. The inhibition of expression of the TDH cells could not be attributed to the Cryptococcus neoformans-specific suppressor T (Ts) cells, even though the Ts cells were induced in CsA-treated mice. Instead, the suppressed expression of the TDH cells in CsA-treated mice was a direct effect of CsA or its products. Our studies with CsA also resulted in the first identification of a population of cells that significantly amplify the anticryptococcal DTH response. The amplifier cells were induced in mice that were given a primary immunizing dose of cryptococcal antigen in complete Freund adjuvant, and they amplified the anticryptococcal DTH response in recipient mice when they were transferred at the time of immunization of the recipient. The amplifier cell population was distinct from the TDH cells in that CsA inhibited the production of the amplifying cells but did not affect the induction of TDH cells. Amplification of the DTH response was a cell-mediated event, since cells but not serum from immunized mice mediated the amplified response in recipient mice. Thus, CsA enabled us to characterize anticryptococcal TDH and Ts cells further and to add to the immune cell circuit of the cryptococcal system a distinct population of cells that amplifies the anticryptococcal DTH response.
环孢素A（CsA）是一种强效免疫抑制药物，被用于进一步探究参与对新型隐球菌抗原迟发型超敏反应（DTH）的淋巴细胞的诱导、表达及调控。我们发现，负责DTH的细胞（TDH细胞）的诱导不受CsA影响，但其表达在经CsA处理的小鼠中受到抑制。TDH细胞表达的抑制不能归因于新型隐球菌特异性抑制性T细胞（Ts细胞），尽管在经CsA处理的小鼠中诱导出了Ts细胞。相反，经CsA处理的小鼠中TDH细胞表达的受抑制是CsA或其产物的直接作用。我们对CsA的研究还首次鉴定出了一群能显著增强抗新型隐球菌DTH反应的细胞。这些增强细胞在接受完全弗氏佐剂中初次免疫剂量新型隐球菌抗原的小鼠中被诱导产生，当在受体小鼠免疫时转移这些增强细胞，它们能增强受体小鼠的抗新型隐球菌DTH反应。增强细胞群体与TDH细胞不同，因为CsA抑制增强细胞的产生，但不影响TDH细胞的诱导。DTH反应的增强是一个细胞介导的事件，因为来自免疫小鼠的细胞而非血清介导了受体小鼠中的增强反应。因此，CsA使我们能够进一步表征抗新型隐球菌的TDH和Ts细胞，并在新型隐球菌系统的免疫细胞回路中增加一群能增强抗新型隐球菌DTH反应的独特细胞。
