分离和鉴定 11 个 Phyllanthaceae 科 Glochidion acuminatum 的微卫星标记。
Isolation and characterization of 11 microsatellite markers for Glochidion acuminatum (Phyllanthaceae).
机构信息
Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan.
Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan ; Japan Science and Technology Agency, Precursory Research for Embryonic Science and Technology (PRESTO), Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan.
出版信息
Appl Plant Sci. 2014 Sep 5;2(9). doi: 10.3732/apps.1400045. eCollection 2014 Sep.
PREMISE OF THE STUDY
Microsatellite markers were developed for Glochidion acuminatum (Phyllanthaceae) to investigate pollen dispersal distances and thereby to assess the effectiveness of specialized Epicephala moths as pollinators. •
METHODS AND RESULTS
Using next-generation sequencing, 11 polymorphic microsatellite primer pairs were developed for G. acuminatum. The primer pairs were tested on 49 individuals from two populations in Japan. The number of alleles per locus ranged from two to 13, and the expected heterozygosity ranged from 0.12 to 0.85. Probability of identity for all loci combined was lower than 1.27 × 10(7). •
CONCLUSIONS
The microsatellite markers developed in this study will be useful for evaluating the benefit of specialized Epicephala moth pollination to Glochidion plants.
研究前提
为 Phyllanthaceae 科的 Glochidion acuminatum 开发了微卫星标记，以研究花粉的散布距离，从而评估专门的 Epicephala 飞蛾作为传粉媒介的有效性。
方法和结果
使用下一代测序技术，为 G. acuminatum 开发了 11 对多态性微卫星引物。这些引物在来自日本两个种群的 49 个个体上进行了测试。每个位点的等位基因数范围从 2 到 13，预期杂合度范围从 0.12 到 0.85。所有位点组合的身份概率均低于 1.27×10(7)。
结论
本研究开发的微卫星标记将有助于评估专门的 Epicephala 飞蛾对 Glochidion 植物传粉的益处。
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