Haroon Javeria, Foureaux Giselle, Martins Almir S, Ferreira Anderson J, Reis Adelina M, Javed Qamar
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Department of Molecular Biology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Department of Morphology, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil; Department of Physiology and Biophysics, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
Department of Morphology, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
Cytokine. 2015 Jan;71(1):54-9. doi: 10.1016/j.cyto.2014.08.008. Epub 2014 Sep 16.
This study examined the sex differences for physical, morphological, histological, mRNA, and protein expression levels changes for interleukins and natriuretic peptides in left ventricle (LV) of two groups of adult FVB/N mice; males (WM) and females (WF). LV morphological, histological, reverse transcription and quantitative real-time PCR (RT-PCR), and immunohistochemical (IHC) alterations were determined in FVB/N mice at 34-35 weeks on gender basis. Confirming the gender dimorphism, FVB/N males (WM) illustrated a significant reduction in ANP and IL1-A levels as well as significantly increased body weight (BW (gm)), tibia length (TL (mm)), heart weight (HW (mg)), heart weight-to-body weight (HW/BW (mg/gm)) ratio, heart weight-to-tibia length (HW/TL (mg/mm)) ratio, left ventricle weight (LV (mg)), left ventricle-to-body weight (LV/BW (mg/gm)) ratio, and left ventricle-to-tibia length (LV/TL (mg/mm)) ratio, left ventricular (LV) cardiomyocyte diameter, high BNP, NPRA, IL-1B, and IL1R1 expression in comparison with FVB/N females (WF). Gender differences in relation to left ventricle (LV) may be due to differences in the interleukins and natriuretic peptides levels as an outcome of sex related hormones.
本研究检测了两组成年FVB/N小鼠（雄性（WM）和雌性（WF））左心室（LV）中白细胞介素和利钠肽在生理、形态、组织学、mRNA和蛋白质表达水平变化的性别差异。基于性别对34 - 35周龄的FVB/N小鼠进行LV形态学、组织学、逆转录和定量实时PCR（RT-PCR）以及免疫组织化学（IHC）改变的测定。证实了性别二态性，FVB/N雄性（WM）的心房钠尿肽（ANP）和白细胞介素1 - A（IL1 - A）水平显著降低，而体重（BW（克））、胫骨长度（TL（毫米））、心脏重量（HW（毫克））、心脏重量与体重之比（HW/BW（毫克/克））、心脏重量与胫骨长度之比（HW/TL（毫克/毫米））、左心室重量（LV（毫克））、左心室与体重之比（LV/BW（毫克/克））以及左心室与胫骨长度之比（LV/TL（毫克/毫米））、左心室（LV）心肌细胞直径、脑钠肽（BNP）、利钠肽受体A（NPRA）、白细胞介素 - 1β（IL - 1B）和白细胞介素1受体1（IL1R1）表达显著增加，与FVB/N雌性（WF）相比。与左心室（LV）相关的性别差异可能是由于与性别相关激素导致的白细胞介素和利钠肽水平差异所致。
