Fan Shengli, Yu Mei, Wang Yigang, Jiang Gangyi
Faculty of Information Science and Engineering, Ningbo University, Ningbo 315211, China School of Information Science and Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
Faculty of Information Science and Engineering, Ningbo University, Ningbo 315211, China.
Biomed Mater Eng. 2014;24(6):2743-9. doi: 10.3233/BME-141092.
Stereo light microscopes (SLM) with narrow vision and shallow depth of field are widely used in micro-domain research. In this paper, we propose a depth estimation method of micro objects based on geometric transformation. By analyzing the optical imaging geometry, the definition of geometric transformation distance is given and the depth-distance relation express is obtained. The parameters of geometric transformation and express are calibrated with calibration board images captured in aid of precise motorized stage. The depth of micro object can be estimated by calculating the geometric transformation distance. The proposed depth-distance relation express is verified using an experiment in which the depth map of an Olanzapine tablet surface is reconstructed.
视野狭窄、景深较浅的立体光学显微镜（SLM）广泛应用于微观领域研究。本文提出一种基于几何变换的微观物体深度估计方法。通过分析光学成像几何，给出几何变换距离的定义并得到深度-距离关系表达式。利用精确电动载物台采集的标定板图像对标定几何变换参数及表达式。通过计算几何变换距离可估计微观物体的深度。通过重建奥氮平片剂表面深度图的实验验证了所提出的深度-距离关系表达式。
