深海海绵扁平多孔海绵（星骨海绵纲，火山海绵科）的完整线粒体基因组。
The complete mitochondrial genome of the deep-sea sponge Poecillastra laminaris (Astrophorida, Vulcanellidae).
作者信息
Zeng Cong, Thomas Leighton J, Kelly Michelle, Gardner Jonathan P A
机构信息
a School of Biological Sciences, Victoria University of Wellington , Wellington , New Zealand and.
b Coasts and Oceans National Centre, National Institute of Water and Atmospheric Research Limited , Auckland , New Zealand.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1658-9. doi: 10.3109/19401736.2014.958716. Epub 2014 Sep 17.
The complete mitochondrial genome of a New Zealand specimen of the deep-sea sponge Poecillastra laminaris (Sollas, 1886) (Astrophorida, Vulcanellidae), from the Colville Ridge, New Zealand, was sequenced using the 454 Life Science pyrosequencing system. To identify homologous mitochondrial sequences, the 454 reads were mapped to the complete mitochondrial genome sequence of Geodia neptuni (GeneBank No. NC_006990). The P. laminaris genome is 18,413 bp in length and includes 14 protein-coding genes, 24 transfer RNA genes and 2 ribosomal RNA genes. Gene order resembled that of other demosponges. The base composition of the genome is A (29.1%), T (35.2%), C (14.0%) and G (21.7%). This is the second published mitogenome for a sponge of the order Astrophorida and will be useful in future phylogenetic analysis of deep-sea sponges.
利用454生命科学焦磷酸测序系统，对采自新西兰科尔维尔海岭的深海海绵波氏层孔海绵（Poecillastra laminaris，索拉斯，1886年）（星状海绵目，火山海绵科）的一个新西兰标本的完整线粒体基因组进行了测序。为了鉴定同源线粒体序列，将454测序读数比对到了海神绵（Geodia neptuni）的完整线粒体基因组序列（基因库编号NC_006990）。波氏层孔海绵的基因组长度为18413 bp，包括14个蛋白质编码基因、24个转移RNA基因和2个核糖体RNA基因。基因排列顺序与其他寻常海绵纲动物相似。基因组的碱基组成为：A（29.1%）、T（35.2%）、C（14.0%）和G（21.7%）。这是星状海绵目海绵的第二个已发表的线粒体基因组，将有助于未来对深海海绵进行系统发育分析。
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