Zeng Cong, Tracey Di M, Clark Malcolm R, Rowden Ashley A, Thomas Leighton J, Gardner Jonathan P A
a School of Biological Sciences, Victoria University of Wellington , Wellington , New Zealand and.
b National Institute of Water and Atmospheric Research Limited , Wellington , New Zealand.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1959-60. doi: 10.3109/19401736.2014.971306. Epub 2014 Oct 20.
Three individual-specific DNA libraries of the deep-sea scleractinian coral Solenosmilia variabilis (Duncan, 1873) were constructed to obtain complete mitochondrial genomes using the 454 Life Science pyrosequencing system. Two mitogenomes were successfully assembled: both were 15,968 bp in length, with base composition of A (24.2%), T (41.1%), C (13.7%) and G (21.0%). The genome contains 13 protein-coding genes, 2 ribosomal RNA genes, 2 transfer RNA genes and a D-loop region. The two mitogenomes were almost identical, with only 5 nucleotide differences (0.03%), including a synonymous substitution within the nad1, nad2 and nad4L genes, and two transversions in the D-loop region. This inter-individual variation indicates that these genes and/or region are potential candidates as molecular markers for population genetic research. The mitogenome of S. variabilis will be useful for future phylogenetic and phylogeographic studies of deep-sea corals.
构建了深海石珊瑚可变索氏珊瑚（Solenosmilia variabilis，邓肯，1873年）的三个个体特异性DNA文库，以使用454生命科学焦磷酸测序系统获得完整的线粒体基因组。成功组装了两个线粒体基因组：两者长度均为15,968 bp，碱基组成为A（24.2%）、T（41.1%）、C（13.7%）和G（21.0%）。该基因组包含13个蛋白质编码基因、2个核糖体RNA基因、2个转移RNA基因和一个D环区域。这两个线粒体基因组几乎相同，只有5个核苷酸差异（0.03%），包括nad1、nad2和nad4L基因内的一个同义替换，以及D环区域的两个颠换。这种个体间变异表明，这些基因和/或区域是群体遗传研究中作为分子标记的潜在候选者。可变索氏珊瑚的线粒体基因组将有助于未来深海珊瑚的系统发育和系统地理学研究。
