Hu Yang, Ji Jie, Ling Fei, Chen Yunhe, Lu Lin, Zhang Qizhong, Wang Gaoxue
a College of Science, Northwest A&F University , Yangling 712100 , China.
J Aquat Anim Health. 2014 Sep;26(3):127-36. doi: 10.1080/08997659.2014.902872.
Abstract Methanol extracts of 24 traditional medicinal plants with potential anthelmintic activity against Dactylogyrus intermedius (Monogenea) in Goldfish Carassius auratus were investigated. Abrus cantoniensis, Citrus medica, Dioscorea collettii, and Polygonum multiflorum exhibited 100% activity and were selected for further evaluation by applying five solvents (petroleum ether, chloroform, ethyl acetate, methanol, and water) for the extraction of the samples, followed by an in vivo bioassay. Among the plants tested, water, methanol, and ethyl-acetate extracts of P. multiflorum showed the highest efficacies; EC50 values (median concentration that results in 50% of its maximal effect) were 1.9, 5.4, and 9.1 mg/L, respectively, and extracts showed 100% efficacy against Dactylogyrus intermedius at 100, 12.5, and 25 mg/L. This was followed by ethyl-acetate, chloroform, and methanol extracts of Dioscorea collettii, which demonstrated 100% efficacy at 80, 80, and 120 mg/L and had EC50 values of 19.7, 27.1, and 37.8 mg/L, respectively, after 48 h of exposure. Chloroform and ethyl-acetate extracts of C. medica, which exhibited 100% efficacy against Dactylogyrus intermedius at 100 and 125 mg/L, revealed similar activity and had EC50 values of 58.7 and 51.3 mg/L, respectively. The ethyl-acetate and methanol extracts of A. cantoniensis exhibited the lowest activity and had EC50 values of 279.4 and 64.3 mg/L. Acute toxicities of these active extracts were investigated on Goldfish for 48 h. The findings indicated that extracts of the four plants can be developed as a preferred natural antiparasitic for the control of D. intermedius. Received June 15, 2013; accepted February 11, 2014.
摘要 对24种具有潜在抗蠕虫活性的传统药用植物的甲醇提取物进行了研究，这些植物对金鱼鲫（鲤科）体内的中型指环虫（单殖吸虫）具有活性。鸡骨草、枸橼、薯蓣和何首乌表现出100%的活性，并通过使用五种溶剂（石油醚、氯仿、乙酸乙酯、甲醇和水）对样品进行萃取，随后进行体内生物测定，从而被选作进一步评估。在所测试的植物中，何首乌的水提取物、甲醇提取物和乙酸乙酯提取物显示出最高的效力；半数效应浓度（EC50值，即导致其最大效应50%的浓度中值）分别为1.9、5.4和9.1毫克/升，提取物在100、12.5和二十五毫克/升时对中型指环虫的效力为100%。其次是薯蓣的乙酸乙酯提取物、氯仿提取物和甲醇提取物，在暴露48小时后，它们在80、80和120毫克/升时表现出100%的效力，EC50值分别为19.7、27.1和37.8毫克/升。枸橼的氯仿提取物和乙酸乙酯提取物在100和125毫克/升时对中型指环虫表现出100%的效力，显示出相似的活性，EC50值分别为58.7和51.3毫克/升。鸡骨草的乙酸乙酯提取物和甲醇提取物表现出最低的活性，EC50值分别为279.4和64.3毫克/升。对金鱼进行了48小时的这些活性提取物的急性毒性研究。研究结果表明，这四种植物的提取物可被开发为控制中型指环虫的首选天然抗寄生虫药物。2013年6月15日收到；2014年2月11日接受。
