Fujii Sotaro, Masanari Misa, Yamanaka Masaru, Wakai Satoshi, Sambongi Yoshihiro
a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
Biosci Biotechnol Biochem. 2014;78(7):1191-4. doi: 10.1080/09168451.2014.912120. Epub 2014 May 28.
Apo-cytochomes c without heme are usually unstructured. Here we showed that apo-form of thermophilic Hydrogenophilus thermoluteolus cytochrome c' (PHCP) was a monomeric protein with high helix content. Apo-PHCP was thermally stable, possibly due to the hydrophobic residues and ion pairs. PHCP is the first example of a structured apo-cytochrome c', which will expand our view of hemoprotein structure formation.
没有血红素的脱辅基细胞色素c通常是无结构的。在此我们表明，嗜热栖热放线菌细胞色素c'（PHCP）的脱辅基形式是一种具有高螺旋含量的单体蛋白。脱辅基PHCP具有热稳定性，这可能归因于疏水残基和离子对。PHCP是结构化脱辅基细胞色素c'的首个实例，这将拓宽我们对血红蛋白结构形成的认识。
