McMillen M A, Schaefer H A, MacArthur J D, Adrian T A, Modlin I M
Department of Surgery, Yale University, New Haven, Connecticut 06520.
J Surg Res. 1989 Apr;46(4):292-5. doi: 10.1016/0022-4804(89)90189-3.
T helper lymphocyte activation is thought to occur when the T3T1 receptor is activated by antigen, and a calcium signal and stimulus to protein kinase C appear to be essential for interleukin-2 production and lymphocyte proliferation. Previous work from our lab has demonstrated that the calcium signal is unaffected by cyclosporine. In this report, a macrophage and T suppressor/cytotoxic-depleted population of human peripheral blood mononuclear cells is stimulated with Sepharose beads bound to OKT3 monoclonal antibody and Sepharose-OKT3 plus a phorbol ester (a stimulus to protein kinase C). Cyclosporine inhibits both the Sepharose/OKT3-mediated and Sepharose/OKT3/phorbol myristic acetate-mediated mitogenesis. Cyclosporine inhibits either protein kinase C or protein kinase C-dependent intracellular signals necessary for T helper activation and proliferation.
人们认为，当T3T1受体被抗原激活时，辅助性T淋巴细胞就会被激活，而钙信号和对蛋白激酶C的刺激似乎是白细胞介素-2产生和淋巴细胞增殖所必需的。我们实验室之前的研究表明，钙信号不受环孢素的影响。在本报告中，用与OKT3单克隆抗体结合的琼脂糖珠以及琼脂糖-OKT3加佛波酯（一种对蛋白激酶C的刺激物）刺激人外周血单个核细胞中巨噬细胞和去除了抑制性/细胞毒性T细胞的群体。环孢素抑制琼脂糖/OKT3介导的和琼脂糖/OKT3/佛波肉豆蔻酸酯介导的有丝分裂。环孢素抑制蛋白激酶C或辅助性T细胞激活和增殖所需的蛋白激酶C依赖性细胞内信号。
