College of Veterinary Medicine, Jilin University, Changchun, 130062, Jilin Province, China.
Inflammation. 2015 Feb;38(1):152-8. doi: 10.1007/s10753-014-0017-9.
Selenium, in the form of selenoproteins, plays a pivotal role in anti-inflammatory processes and antioxidant defense system. The aim of this study was to examine the effects of selenium on lipopolysaccharide (LPS)-induced inflammatory responses in bovine mammary epithelial cells (bMEC) and to investigate the potential mechanism. bMEC viability was measured by MTT assay. TNF-α, IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) messenger RNA (mRNA) expressions were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The activation of nuclear factor-kappa B (NF-κB) was determined by Western blotting. The results showed that the mRNA expressions of these inflammatory factors were significantly inhibited by selenium in a dose-dependent manner. At protein levels, Western blot analysis demonstrated that selenium dose-dependently decreased NF-κB p65 translocating from the cytoplasm to the nucleus. Taken together, these results suggest that the anti-inflammatory property of selenium in LPS-stimulated primary bMEC may be attributed to the downregulation of NF-κB activation.
硒以硒蛋白的形式发挥作用，在抗炎过程和抗氧化防御系统中发挥关键作用。本研究旨在探讨硒对脂多糖（LPS）诱导的奶牛乳腺上皮细胞（bMEC）炎症反应的影响，并探讨其潜在机制。MTT 法测定细胞活力。通过实时定量聚合酶链反应（qRT-PCR）评估 TNF-α、IL-1β、环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）信使 RNA（mRNA）的表达。通过 Western blot 测定核因子-κB（NF-κB）的激活。结果表明，硒以剂量依赖性方式显著抑制这些炎症因子的 mRNA 表达。在蛋白质水平上，Western blot 分析表明，硒可剂量依赖性地减少 NF-κB p65 从细胞质向细胞核的易位。综上所述，这些结果表明，硒对 LPS 刺激的原代 bMEC 的抗炎作用可能归因于 NF-κB 激活的下调。
