Lu Zhichuang, Zhang Nan, Song Na, Gao Tianxiang
a Institute of Evolution and Marine Biodiversity, Ocean University of China , Qingdao 266003 , People's Republic of China .
b Key Laboratory of Marine Biological Resources and Ecology , Liaoning Ocean and Fisheries Science Research Institute , Dalian 116023 , People's Republic of China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1662-3. doi: 10.3109/19401736.2014.958718. Epub 2014 Sep 18.
In this study, the complete mitochondrial genome (mitogenome) sequence of Lota lota has been determined by long polymerase chain reaction and primer walking methods. The mitogenome is a circular molecule of 16,519 bp in length and contains 37 mitochondrial genes including 13 protein-coding genes, 2 ribosomal RNA (rRNA), 22 transfer RNA (tRNA) and a control region as other bony fishes. Within the control region, we identified the termination-associated sequence domain (TAS), the central conserved sequence block domains (CSB-F and CSB-D), and the conserved sequence block domains (CSB-1, CSB-2 and CSB-3).
在本研究中，通过长聚合酶链反应和引物步移法确定了江鳕的完整线粒体基因组（线粒体基因组）序列。该线粒体基因组是一个长度为16,519 bp的环状分子，与其他硬骨鱼类一样，包含37个线粒体基因，包括13个蛋白质编码基因、2个核糖体RNA（rRNA）、22个转运RNA（tRNA）和一个控制区域。在控制区域内，我们鉴定出了终止相关序列结构域（TAS）、中央保守序列块结构域（CSB-F和CSB-D）以及保守序列块结构域（CSB-1、CSB-2和CSB-3）。
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