Wang Yanping, Song Na, Sun Dianrong, Gao Tianxiang
a Institute of Evolution and Marine Biodiversity, Ocean University of China , Qingdao , People's Republic of China and.
b South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , People's Republic of China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):393-4. doi: 10.3109/19401736.2014.896001. Epub 2014 Mar 24.
In this study, the complete mitochondrial genome (mitogenome) sequence of Saurida microlepis has been determined by long PCR and primer walking methods. The complete mitochondrial genome is a circular molecule of 16,510 bp in length and contains the same set of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA (rRNA), 22 transfer RNA (tRNA)), and a control region as other bony fishes. Within the control region, we identified the termination-associated sequence domain (TAS), the central conserved sequence block domains (CSB-F, CSB-E, CSB-D, CSB-C, CSB-B and CSB-A), and the conserved sequence block domains (CSB-1, CSB-2 and CSB-3).
在本研究中,已通过长PCR和引物步移法确定了小鳞蛇鲻的完整线粒体基因组(线粒体基因组)序列。完整的线粒体基因组是一个长度为16,510 bp的环状分子,与其他硬骨鱼类一样,包含一组相同的37个线粒体基因(13个蛋白质编码基因、2个核糖体RNA(rRNA)、22个转移RNA(tRNA))以及一个控制区。在控制区内,我们鉴定出了终止相关序列结构域(TAS)、中央保守序列块结构域(CSB-F、CSB-E、CSB-D、CSB-C、CSB-B和CSB-A)以及保守序列块结构域(CSB-1、CSB-2和CSB-3)。