Zhang Yuanyuan, Yu Wansong, Pei Lu, Lai Keqiang, Rasco Barbara A, Huang Yiqun
College of Food Science and Technology, Shanghai Ocean University, No. 999 Hucheng Huan Road, LinGang New City, Shanghai 201306, China.
School of Food Science, Washington State University, Pullman, WA 99164-6376, USA.
Food Chem. 2015 Feb 15;169:80-4. doi: 10.1016/j.foodchem.2014.07.129. Epub 2014 Aug 7.
Surface-enhanced resonance Raman scattering (SERRS) coupled with gold nanospheres was applied for rapid analysis of the hazardous substances malachite green (MG) and leucomalachite green (LMG) in fish muscle tissues. The lowest concentration of MG that could be detected was 0.5ngmL(-1) with high linear correlation (R(2)=0.970-0.998) between MG concentration and intensities of characteristic Raman peaks. A simplified sample preparation method taking less than 1h for recovering MG and LMG in fish fillets was developed for SERRS analysis, and 4-8 samples could be handled in parallel. MG and LMG could be detected in extracts of tilapia fish fillets at as low as 2ngg(-1) with SERRS and a simple principle component analysis method. For six other fish species, the lowest detectable concentration of MG ranged from 1ngg(-1) to 10ngg(-1). This study provides a new sensitive approach for the detection of trace amounts of the prohibited drugs MG and LMG in muscle food, which has the potential for rapidly screening a large number of samples.
采用金纳米球耦合的表面增强共振拉曼散射（SERRS）技术对鱼肌肉组织中的有害物质孔雀石绿（MG）和无色孔雀石绿（LMG）进行快速分析。可检测到的MG最低浓度为0.5ngmL(-1)，MG浓度与特征拉曼峰强度之间具有高度线性相关性（R(2)=0.970 - 0.998）。开发了一种简化的样品制备方法，用于在鱼片中回收MG和LMG以便进行SERRS分析，该方法耗时不到1小时，并且可以并行处理4 - 8个样品。利用SERRS和一种简单的主成分分析方法，可在罗非鱼鱼片提取物中检测到低至2ngg(-1)的MG和LMG。对于其他六种鱼类，MG的最低可检测浓度范围为1ngg(-1)至10ngg(-1)。本研究为检测肌肉类食品中痕量违禁药物MG和LMG提供了一种新的灵敏方法，具有快速筛查大量样品的潜力。
