Jouyban Abolghasem, Hasanzadeh Mohammad, Shadjou Nasrin
Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
Iran J Pharm Res. 2014 Spring;13(2):471-86.
A capillary electrophoresis method was used for assay of some degradation products of carvedilol. The optimized parameters were as; running buffer 80 mM acetate dissolved in methanol/ethanol mixture (65:35% v/v), applied voltage of 19 kV, temperature is 20 ºC and the wavelength range of 200-350 nm. The results indicate that the proposed capillary electrophoresis method could effectively separate carvedilol from its degradation products and can be employed as a stability indicating assay method. In addition, the presence of a new unknown degradation product was discovered by this method. In addition, capillary electrophoresis behaviour of carvedilol in photo/force degradation conditions gave valuable information concerning the dissimilarities of their ionization. Results indicated that the capillary electrophoresis proposed method can be used for the determination of carvedilol in human serum. Finally, accuracy of the proposed method was established by recovery experiments from spiked human serum samples.
采用毛细管电泳法测定卡维地洛的一些降解产物。优化参数如下：运行缓冲液为80 mM乙酸盐溶解于甲醇/乙醇混合液（65:35% v/v），施加电压19 kV，温度20 ºC，波长范围200 - 350 nm。结果表明，所提出的毛细管电泳法能够有效分离卡维地洛及其降解产物，可作为一种稳定性指示测定方法。此外，通过该方法发现了一种新的未知降解产物。另外，卡维地洛在光/力降解条件下的毛细管电泳行为提供了有关其电离差异的有价值信息。结果表明，所提出的毛细管电泳法可用于测定人血清中的卡维地洛。最后，通过加标人血清样品的回收实验确定了该方法的准确性。
