Keyhanmanesh Rana, Nazemiyeh Hossein, Mazouchian Hossein, Bagheri Asl Mohammad Mahdi, Karimi Shoar Mahdi, Alipour Mohammad Reza, Boskabady Mohammad Hossein
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran ; Department of Physiology, Tabriz University of Medical Sciences, Tabriz, IR Iran ; Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
Pharmaceutical Nanotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
Iran Red Crescent Med J. 2014 Jul;16(7):e10421. doi: 10.5812/ircmj.10421. Epub 2014 Jul 5.
In previous studies, the bronchodilator and antitussive effects of Nigella sativa have been demonstrated on guinea pigs.
In the present study, the effect of the hydroethanolic extract of N. sativa on tracheal responsiveness in guinea pigs exposed to cigarette smoke was examined.
Three groups of guinea pig models of COPD were given drinking water alone (COPD group), drinking water containing vitamin C (COPD + VC group), and N. sativa (COPD + NS group). Tracheal responses to methacholine were measured as effective concentration causing 50% of maximum response (EC50 M) in control animals (group C) and three groups of guinea pigs with COPD (n = 7, for all groups). Tracheal responses to 0.1% ovalbumin in comparison to contraction obtained by 10 µM methacholine were also examined.
The tracheal responsiveness to both methacholine and ovalbumin in guinea pigs with COPD were significantly higher than those of controls (P < 0.001 for both cases). The tracheal responsiveness in the COPD + VC and the COPD + NS groups to both methacholine and ovalbumin were significantly decreased in comparison to the COPD group (P < 0.05 and P < 0.001, respectively).
These results showed the preventive effect of hydroethanolic extract of N. sativa on tracheal responsiveness of guinea pig model of COPD, which was as effective as vitamin C.
在先前的研究中，已证实黑种草对豚鼠具有支气管扩张和镇咳作用。
在本研究中，检测了黑种草水乙醇提取物对暴露于香烟烟雾的豚鼠气管反应性的影响。
三组慢性阻塞性肺疾病（COPD）豚鼠模型分别给予单纯饮用水（COPD组）、含维生素C的饮用水（COPD + VC组）和黑种草（COPD + NS组）。测量气管对乙酰甲胆碱的反应，以对照动物（C组）和三组COPD豚鼠（每组n = 7）中引起最大反应50%的有效浓度（EC50 M）表示。还检测了气管对0.1%卵清蛋白的反应，并与10 µM乙酰甲胆碱引起的收缩反应进行比较。
COPD豚鼠对乙酰甲胆碱和卵清蛋白的气管反应性均显著高于对照组（两种情况P均< 0.001）。与COPD组相比，COPD + VC组和COPD + NS组对乙酰甲胆碱和卵清蛋白的气管反应性均显著降低（分别为P < 0.05和P < 0.001）。
这些结果表明黑种草水乙醇提取物对COPD豚鼠模型的气管反应性具有预防作用，其效果与维生素C相当。
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