Haines Margaret L, Moussalli Adnan, Stuart-Fox Devi, Clemann Nick, Melville Jane
Sciences Department, Museum Victoria, GPO Box 666, Melbourne, VIC 3001, Australia; Zoology Department, University of Melbourne, Parkville, VIC 3010, Australia.
Sciences Department, Museum Victoria, GPO Box 666, Melbourne, VIC 3001, Australia.
Mol Phylogenet Evol. 2014 Dec;81:86-95. doi: 10.1016/j.ympev.2014.09.006. Epub 2014 Sep 19.
The Australian scincid genus Pseudemoia comprises six morphologically similar species restricted to temperate south-eastern Australia. Due to the high degree of morphological conservatism, phylogenetic relationships and taxonomic status within the Pseudemoia entrecasteauxii complex (comprising the nominal species P. entrecasteauxii, P. cryodroma, and P. pagenstecheri) remains unresolved. To further investigate the phylogenetic relationships and taxonomic status of Pseudemoia spp., and to test the hypothesis that P. cryodroma evolved from hybridization between P. entrecasteauxii and P. pagenstecheri, we sequenced one mitochondrial locus (ND4) and five nuclear loci (β-globin, LGMN, PRLR, Rhodopsin, RPS8). While we find strong support for the monophyly of the P. entrecasteauxii complex, there exists marked incongruence between the mitochondrial and nuclear markers, particularly in regards to the high altitude specialist, P. cryodroma. The most parsimonious explanation of this discordance is historic mitochondrial introgression, although a hybrid origin for P. cryodroma cannot be completely rejected. Within P. pagenstecheri sensu lato, we identified a strongly supported, highly divergent yet morphologically cryptic lineage restricted to northern New South Wales. Although more weakly supported by the nuDNA, we also identified a second geographically distinct lineage of P. pagenstecheri s.l., which may warrant separate conservation management. Our study reveals a more complex evolutionary history of the genus Pseudemoia than previously appreciated and contributes to our understanding of the biogeography and evolution of Australian mesic zone fauna.
澳大利亚石龙子属（Pseudemoia）包含六个形态相似的物种，仅分布于澳大利亚东南部温带地区。由于高度的形态保守性，在Entrecasteaux石龙子复合体（Pseudemoia entrecasteauxii complex，包括指名物种P. entrecasteauxii、P. cryodroma和P. pagenstecheri）内的系统发育关系和分类地位仍未得到解决。为了进一步研究Pseudemoia属物种的系统发育关系和分类地位，并检验P. cryodroma是由P. entrecasteauxii和P. pagenstecheri杂交演化而来的假说，我们对一个线粒体基因座（ND4）和五个核基因座（β - 珠蛋白、LGMN、PRLR、视紫红质、RPS8）进行了测序。虽然我们强烈支持Entrecasteaux石龙子复合体的单系性，但线粒体和核标记之间存在明显的不一致，特别是对于高海拔特化物种P. cryodroma。对这种不一致最简约的解释是历史上的线粒体基因渗入，尽管不能完全排除P. cryodroma的杂交起源。在广义的P. pagenstecheri范围内，我们确定了一个得到强烈支持、高度分化但形态上难以区分的谱系，该谱系仅分布于新南威尔士州北部。虽然由核DNA提供的支持较弱，但我们还确定了广义P. pagenstecheri的第二个地理上不同的谱系，这可能需要单独的保护管理。我们的研究揭示了Pseudemoia属比以前所认识到的更为复杂的进化历史，并有助于我们理解澳大利亚中生带动物区系的生物地理学和进化。
