Shen Zhisen, Li Qun, Deng Hongxia, Lu Dakai, Song Haojun, Guo Junming
Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of Ningbo University, Ningbo, China.
Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of Ningbo University, Ningbo, China; Ningbo University School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo, China.
PLoS One. 2014 Sep 22;9(9):e108237. doi: 10.1371/journal.pone.0108237. eCollection 2014.
Long non-coding RNAs (lncRNAs) are novel transcripts that may play important roles in cancer. Our study aimed to resolve the lncRNA profile of larynx squamous cell carcinoma (LSCC) and to determine its clinical significance. The global lncRNA expression profile in LSCC tissues was measured by lncRNA microarray. Distinctly expressed lncRNAs were identified and levels of AC026166.2-001 and RP11-169D4.1-001 lncRNAs in 87 LSCC samples and paired adjacent normal tissue were analyzed by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The clinical significance of these lncRNAs in laryngeal cancer was analyzed and survival data were estimated by the Kaplan-Meier method and the log-rank test. A receiver operating characteristic (ROC) curve was constructed to check the diagnostic value. In the lncRNA expression profile of tumor samples, 684 lncRNAs were upregulated and 747 lncRNAs were downregulated (fold-change >2.0). Of these, AC026166.2-001 and RP11-169D4.1-001 were distinctly dysregulated, with AC026166.2-001 exhibiting lower expression in cancer tissues and RP11-169D4.1-001 higher expression. We verified that both AC026166.2-001 and RP11-169D4.1-001 were expressed at a lower level in cervical lymph nodes compared with paired laryngeal cancer tissues and paired normal tissues. RP11-169D4.1-001 levels were positively correlated with lymph node metastasis (P = 0.007). From the survival analysis, decreased levels of AC026166.2-001 and RP11-169D4.1-001 were associated with poorer prognosis. The area under the ROC curve was up to 0.65 and 0.67, respectively, and the cut-off point of ΔCt was 11.23 and 10.53, respectively. AC026166.2-001 and RP11-169D4.1-001 may act as novel biomarkers in LSCC and may be potential therapeutic targets for LSCC patients. Both AC026166.2-001 and RP11-169D4.1-001 could be independent prognostic factors for survival in LSCC.
长链非编码RNA(lncRNAs)是一类新型转录本,可能在癌症中发挥重要作用。我们的研究旨在解析喉鳞状细胞癌(LSCC)的lncRNA图谱,并确定其临床意义。通过lncRNA微阵列检测LSCC组织中的整体lncRNA表达谱。鉴定出差异表达的lncRNAs,并通过实时定量逆转录聚合酶链反应(qRT-PCR)分析87例LSCC样本及配对的相邻正常组织中AC026166.2 - 001和RP11 - 169D4.1 - 001 lncRNAs的水平。分析这些lncRNAs在喉癌中的临床意义,并采用Kaplan-Meier法和对数秩检验估计生存数据。构建受试者工作特征(ROC)曲线以检验诊断价值。在肿瘤样本的lncRNA表达谱中,684个lncRNAs上调,747个lncRNAs下调(变化倍数>2.0)。其中,AC026166.2 - 001和RP11 - 169D4.1 - 001明显失调,AC026166.2 - 001在癌组织中表达较低,而RP11 - 169D4.1 - 001表达较高。我们证实,与配对的喉癌组织和配对的正常组织相比,AC026166.2 - 001和RP11 - 169D4.1 - 001在颈部淋巴结中的表达水平均较低。RP11 - 169D4.1 - 001水平与淋巴结转移呈正相关(P = 0.007)。生存分析显示,AC026166.2 - 001和RP11 - 169D4.1 - 001水平降低与预后较差相关。ROC曲线下面积分别高达0.65和0.67,ΔCt的截断点分别为11.23和10.53。AC026166.2 - 001和RP11 - 169D4.1 - 00可能作为LSCC的新型生物标志物,并且可能是LSCC患者潜在的治疗靶点。AC026166.2 - 001和RP11 - 169D4.1 - 001均可能是LSCC患者生存的独立预后因素。