Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, 315211, China.
Med Oncol. 2013 Dec;30(4):709. doi: 10.1007/s12032-013-0709-2. Epub 2013 Sep 1.
Long noncoding RNAs (lncRNAs) play crucial roles during cancer occurrence and progression. The pseudogene-expressed lncRNA is one major type of lncRNA family. However, their association with cancers is largely unknown. In this study, we focused on small ubiquitin-like modifier (SUMO) 1 pseudogene 3, SUMO1P3. Gastric cancer tissues and adjacent nontumor tissues were collected from 96 patients with gastric cancer. The SUMO1P3 levels were detected by quantitative reverse transcription-polymerase chain reaction. Then, the association between the level of SUMO1P3 in gastric cancer tissues and the clinicopathological features of patients with gastric cancer was further analyzed. A receiver operating characteristic curve was constructed for differentiating patients with gastric cancer from patients with benign gastric diseases. The results showed that SUMO1P3 was significantly up-regulated in gastric cancer tissues compared with paired-adjacent nontumorous tissues (p < 0.01). Its expression level was significantly correlated with tumor size (p = 0.003), differentiation (p = 0.002), lymphatic metastasis (p = 0.001), and invasion (p = 0.039). The area under the ROC curve of SUMO1P3 was up to 0.666. These results indicated, for the first time, that pseudogene-expressed lncRNA SUMO1P3 may be a potential biomarker in the diagnosis of gastric cancer.
长链非编码 RNA（lncRNA）在癌症的发生和发展中起着至关重要的作用。假基因表达的 lncRNA 是 lncRNA 家族的主要类型之一。然而，它们与癌症的关联在很大程度上是未知的。在这项研究中，我们专注于小泛素样修饰物（SUMO）1 假基因 3，SUMO1P3。从 96 名胃癌患者中收集胃癌组织和相邻非肿瘤组织。通过定量逆转录聚合酶链反应检测 SUMO1P3 水平。然后，进一步分析了胃癌组织中 SUMO1P3 水平与胃癌患者临床病理特征之间的关系。构建了一个区分胃癌患者和良性胃部疾病患者的受试者工作特征曲线。结果表明，与配对的相邻非肿瘤组织相比，胃癌组织中 SUMO1P3 显著上调（p<0.01）。其表达水平与肿瘤大小（p=0.003）、分化（p=0.002）、淋巴转移（p=0.001）和浸润（p=0.039）显著相关。SUMO1P3 的 ROC 曲线下面积高达 0.666。这些结果首次表明，假基因表达的 lncRNA SUMO1P3 可能是胃癌诊断的潜在生物标志物。
