脑啡肽酶（EC 3.4.24.11）对表达常见急性淋巴细胞白血病抗原（CALLA）的各种白血病细胞的活性表征。

Characterisation of enkephalinase (EC 3.4.24.11) activity on various leukemic cells expressing the common acute lymphocytic leukemia antigen (CALLA).

作者信息

Arock M, Gros C, Devillier P, Guillosson J J, Schwartz J C

机构信息

Laboratoire d'Hématologie, Faculté de Pharmacie, Paris, France.

出版信息

FEBS Lett. 1989 May 8;248(1-2):123-6. doi: 10.1016/0014-5793(89)80445-4.

DOI:10.1016/0014-5793(89)80445-4

PMID:2524403

Abstract

The deduced amino acid sequences of CALLA, a cell surface marker of human acute lymphocytic leukemia, and human enkephalinase (neutral endopeptidase, EC 3.4.24.11) were recently reported to be almost identical. We show that membranes of CALLA+ cells of the REH lymphoblastic cell line as well as blast cells derived from the blood or bone marrow of patients with acute lymphocytic leukemia display high enkephalinase activity. This activity was abrogated by several enkephalinase inhibitors at concentrations closely similar to those required to inhibit pure human enkephalinase. However, these compounds did not significantly modify the rate of REH cell proliferation in vitro. Hence, the functional role, if any, of the high peptidase activity in lymphoblastic cells remains to be established.

摘要

最近有报道称，人类急性淋巴细胞白血病的细胞表面标志物CALLA与人类脑啡肽酶（中性内肽酶，EC 3.4.24.11）的推导氨基酸序列几乎相同。我们发现，REH淋巴母细胞系的CALLA+细胞的膜以及急性淋巴细胞白血病患者血液或骨髓来源的原始细胞均表现出高脑啡肽酶活性。几种脑啡肽酶抑制剂在与抑制纯人类脑啡肽酶所需浓度非常相似的浓度下可消除这种活性。然而，这些化合物并未显著改变REH细胞在体外的增殖速率。因此，淋巴母细胞中高肽酶活性的功能作用（如果有的话）仍有待确定。