水牛（Bubalus bubalis）精浆中的类钙调蛋白及其对精子Ca++、Mg++-ATP酶的影响。

Calmodulin-like protein in buffalo (Bubalus bubalis) seminal plasma and its effect on sperm Ca++, Mg++-ATPase.

作者信息

Sidhu K S, Guraya S S

机构信息

Department of Zoology, Punjab Agricultural University, Ludhiana, India.

出版信息

Int J Androl. 1989 Apr;12(2):148-54. doi: 10.1111/j.1365-2605.1989.tb01298.x.

DOI:10.1111/j.1365-2605.1989.tb01298.x

PMID:2524448

Abstract

A calmodulin-like protein (CLP) has been identified in buffalo (Bubalus bubalis) seminal plasma and partially characterized. It was heat stable and had properties similar to those of the calcium-binding protein, calmodulin. It is present in relatively high concentrations in buffalo seminal plasma. When added to buffalo red-blood cell plasma membrane (RBC ghosts) it increased Ca++, Mg++-ATPase activity by 112%. The activation is counteracted by chlorpromazine and trifluoperazine, the anti-calmodulin drugs. A similar calmodulin-activated Ca++ pump has been found predominantly in the tail fractions of buffalo spermatozoa. The existence of CLP in buffalo seminal plasma may be responsible for some of the physiological changes observed during capacitation and acrosome reaction. A hypothesis has been proposed involving CLP in regulation of these events.

摘要

在水牛（Bubalus bubalis）精浆中已鉴定出一种类钙调蛋白（CLP），并对其进行了部分表征。它具有热稳定性，且具有与钙结合蛋白钙调蛋白相似的特性。它在水牛精浆中的浓度相对较高。当添加到水牛红细胞质膜（RBC膜泡）中时，它使Ca++、Mg++-ATP酶活性提高了112%。这种激活作用可被抗钙调蛋白药物氯丙嗪和三氟拉嗪抵消。在水牛精子的尾部组分中主要发现了一种类似的钙调蛋白激活的Ca++泵。水牛精浆中CLP的存在可能是获能和顶体反应期间观察到的一些生理变化的原因。有人提出了一个涉及CLP对这些事件进行调节的假说。