对活体和固定的黑腹果蝇胚胎的背腹轴进行成像。

Imaging the dorsal-ventral axis of live and fixed Drosophila melanogaster embryos.

作者信息

Carrell Sophia N, Reeves Gregory T

机构信息

Department of Chemical and Biomolecular Engineering, North Carolina State University, 911 Partners Way, Raleigh, NC, 27695, USA.

出版信息

Methods Mol Biol. 2015;1189:63-78. doi: 10.1007/978-1-4939-1164-6_5.

DOI:10.1007/978-1-4939-1164-6_5

PMID:25245687

Abstract

Optimal imaging conditions are of critical importance in developmental biology, as much of the data in the discipline is acquired through microscopy. However, imaging deep sections of tissue, especially live tissue, can be a technical challenge due to light scattering and difficulties in mounting the sample. In particular, capturing high-quality images of dorsal-ventral cross sections requires "end-on" mounting to orient the anterior-posterior axis vertically. Here we present methods to mount and image dorsal-ventral cross sections of both live and fixed Drosophila melanogaster embryos. Our methods have the advantages of being rapid, allowing deep optical sections, and not requiring expensive, specialized equipment.

摘要

最佳成像条件在发育生物学中至关重要，因为该学科的许多数据是通过显微镜获得的。然而，由于光散射和样品固定困难，对组织的深部切片（尤其是活组织）进行成像可能是一项技术挑战。特别是，获取背腹横截面的高质量图像需要“端对端”固定，以使前后轴垂直定向。在这里，我们展示了对活的和固定的黑腹果蝇胚胎的背腹横截面进行固定和成像的方法。我们的方法具有快速、允许进行深部光学切片且不需要昂贵的专业设备的优点。