通过组织表面张力测定法测量细胞间黏附力。

Measurement of intercellular cohesion by tissue surface tensiometry.

作者信息

Foty Ramsey A

机构信息

Department of Surgery, Rutgers-Robert Wood Johnson Medical School, 125 Paterson Street, CAB 7319, New Brunswick, NJ, 08901, USA,

出版信息

Methods Mol Biol. 2015;1189:237-54. doi: 10.1007/978-1-4939-1164-6_16.

DOI:10.1007/978-1-4939-1164-6_16

PMID:25245698

Abstract

Intercellular adhesion plays a vital role in many biological processes including embryonic development, malignant invasion, and wound healing, and can be manipulated to generate complex structures in tissue engineering applications. Accurate measurement of the strength of intercellular adhesion is not trivial and requires methods rooted in sound physical principles. Tissue surface tensiometry (TST) rigorously quantifies intercellular cohesive energy of 3D tissue-like aggregates under physiological conditions. TST utilizes a custom-built tensiometer to compress 3D spheroids between parallel plates. The resistance to the applied force and changes in aggregate geometry are applied to the Young-Laplace equation, generating a measurement of apparent surface tension. We describe all components comprising the tensiometer and provide step by step instructions of all the key steps involved in generating spherical aggregates. We explain how tissue surface tension is calculated and provide a statistical analysis of a sample data set from 12 aggregates.

摘要

细胞间黏附在许多生物过程中起着至关重要的作用，包括胚胎发育、恶性侵袭和伤口愈合，并且在组织工程应用中可以通过操控来生成复杂结构。准确测量细胞间黏附强度并非易事，需要基于可靠物理原理的方法。组织表面张力测定法（TST）能在生理条件下严格量化三维组织样聚集体的细胞间内聚能。TST利用定制的张力计在平行板之间压缩三维球体。将施加力的阻力和聚集体几何形状的变化应用于杨-拉普拉斯方程，从而得出表观表面张力的测量值。我们描述了构成张力计的所有组件，并提供了生成球形聚集体所涉及的所有关键步骤的分步说明。我们解释了如何计算组织表面张力，并对来自12个聚集体的样本数据集进行了统计分析。