Suo Hairui, Xu Kedi, Zheng Xiaoxiang
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, China Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, China.
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, China Qiushi Academy for Advanced Studies (QAAS), Zhejiang University, Hangzhou, China Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, China
J Biomater Appl. 2015 Feb;29(7):977-87. doi: 10.1177/0885328214551009. Epub 2014 Sep 23.
The use of hydrogel-based cell transport scaffolds holds great promise in regenerative medicine, such as treating osteoarthritis. Gelatin and glucosamine are the ideal materials to be used in the hydrogel scaffolds for cartilage regeneration for they could act as compositions of cartilage. To overcome the weak strength of traditional gelatin hydrogels and down-regulate cell toxicity of glucosamine, gelatin and glucosamine molecules were grafted with acrylate groups and covalently crosslinked under photo-radiation to form hydrogels. Hydrogels with tuning physiochemical properties were produced according to different proportions of methacrylate gelatin (GelMA) and N-acryloyl glucosamine (AGA). The process of photocrosslinking was elaborated, and the hypothesis of increasing AGA concentration leading to higher strength of hydrogels was corroborated by testing rheological property and scanning micro-morphological features. A serial of properties, including smaller swelling ratio, lower gelatin dissolution and slower degradation of GelMA/AGA hydrogels with higher AGA concentration further proved our hypothesis. Moreover, AGA molecules showed less cytotoxicity than unmodified glucosamine molecules and the incorporation of AGA molecules in GelMA/AGA hydrogels upregulated cell adhesion and spreading on the hydrogel surface. All of these results indicated that addition of AGA molecules could significantly alter the physiochemical properties of GelMA/AGA hydrogels, which may have broad application prospects in the future.
基于水凝胶的细胞运输支架在再生医学中具有巨大潜力，例如用于治疗骨关节炎。明胶和氨基葡萄糖是用于软骨再生的水凝胶支架的理想材料，因为它们可以作为软骨的组成成分。为了克服传统明胶水凝胶强度较弱的问题并下调氨基葡萄糖的细胞毒性，将明胶和氨基葡萄糖分子接枝丙烯酸酯基团，并在光辐射下共价交联以形成水凝胶。根据甲基丙烯酸明胶（GelMA）和N-丙烯酰基氨基葡萄糖（AGA）的不同比例制备了具有可调物理化学性质的水凝胶。阐述了光交联过程，并通过测试流变性能和扫描微观形态特征证实了增加AGA浓度会导致水凝胶强度更高的假设。一系列特性，包括较高AGA浓度的GelMA/AGA水凝胶具有更小的溶胀率、更低的明胶溶解率和更慢的降解速度，进一步证明了我们的假设。此外，AGA分子比未修饰的氨基葡萄糖分子表现出更低的细胞毒性，并且在GelMA/AGA水凝胶中加入AGA分子上调了细胞在水凝胶表面的粘附和铺展。所有这些结果表明，添加AGA分子可以显著改变GelMA/AGA水凝胶的物理化学性质，这在未来可能具有广阔的应用前景。
