D'Amato Felicetta, Rouli Laetitia, Edouard Sophie, Tyczka Judith, Million Matthieu, Robert Catherine, Nguyen Thi Tien, Raoult Didier
Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France.
Chemisches und Veterinäruntersuchungsamt Karlsruhe, Weißenburger Straße 3, Abteilung 7, Karlsruhe 76187, Germany.
Comp Immunol Microbiol Infect Dis. 2014 Dec;37(5-6):281-8. doi: 10.1016/j.cimid.2014.08.003. Epub 2014 Sep 16.
Coxiella burnetii is a pathogen causing Q fever. The aim of our work was to study Z3055, a strain that is genotypically related to the strain causing the Netherlands outbreak. We compared Z3055 to 5 other completed genomes available in GenBank. We calculated the blast score ratio (BSR) to analyze genetic differences among the strains. The ratio core genome/pangenome was 98% likely other bacteria with closed pangenomes. Differences between Z3055 and the reference NMI consisted only of point mutations and insertion/deletion (INDELs). Non-synonymous mutations significantly increased in genes coding for membrane proteins (16/156 vs 103/1757, bilateral Chi(2) test, p<0.05), ankyrin repeat domains containing proteins (2/9 vs 117/1904, bilateral Chi(2) test, p<0.05), transcription factors (7/53 vs 112/1860, bilateral Chi(2) test, p<0.05) and translation proteins (15/144 vs 109/1655, bilateral Chi(2) test, p<0.05). The evolution of this strain may have been driven by mutations in critical genes.
伯纳特柯克斯体是一种引起Q热的病原体。我们工作的目的是研究Z3055，这是一种在基因上与导致荷兰疫情爆发的菌株相关的菌株。我们将Z3055与GenBank中其他5个已完成的基因组进行了比较。我们计算了比对得分率（BSR）以分析菌株之间的遗传差异。核心基因组/泛基因组的比率为98%，这与其他具有封闭泛基因组的细菌相似。Z3055与参考菌株NMI之间的差异仅包括点突变和插入/缺失（INDELs）。在编码膜蛋白的基因中，非同义突变显著增加（16/156对103/1757，双侧卡方检验，p<0.05），含锚蛋白重复结构域的蛋白质（2/9对117/1904，双侧卡方检验，p<0.05），转录因子（7/53对112/1860，双侧卡方检验，p<0.05）和翻译蛋白（15/144对109/1655，双侧卡方检验，p<0.05）。该菌株的进化可能是由关键基因的突变驱动的。
